5种海洋微藻细胞膜与细胞内脂肪酸组成分析  

The Fatty Acid Composition in Membrane and Intracellular Components of Five Species of Marine Microalgae

作　　者：孙书勤[1] 王江涛[1] 孙宝维[1] 郑宇[1] 孙铭一[1,2] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100 [2]美国佐治亚大学海洋科学学院,佐治亚306023636

出　　处：《中国海洋大学学报（自然科学版）》2010年第S1期191-196,共6页Periodical of Ocean University of China

基　　金：国家重点基础研究发展计划项目(2001CB409703);国家高技术研究发展计划项目(2006AA09Z176);中国海洋大学绿卡教授项目资助

摘　　要：对5种海洋微藻的脂肪酸组成进行了研究。微藻在确定的条件下生长,分别于指数生长前期和稳定期后期,测定其脂肪酸组成。根据细胞膜上和细胞内脂肪酸极性差别,采用硅胶柱层析法分离脂肪酸,应用GC与GC-MS进行定量与定性分析。实验结果表明:在微藻生长的不同时期,细胞内脂与细胞膜脂含量及各自的脂肪酸组成明显不同;无论是指数生长前期还是稳定期后期,胞内物质的脂肪酸含量明显要低于细胞膜组分,细胞内与细胞膜中主要组成是C16系与C18系脂肪酸。To examine composition of algal fatty acids,five marine microalgae were cultured and harvested at logarithmic and stationary phases.Their membrane and intracellular fatty acids were separated by silica gel column due to the polarity.Quantification and identification of fatty acids were performed on GC and GC-MS.Experimental results showed that the composition and concentration of membrane and intracellular fatty acids were apparently different during the two growth phases.Content of intracellular fatty acids were lower than that of membrane composition at both growth stage,C16 and C18 fatty acids were dominated composition in membrane and intracellular components.

关 键 词：海洋微藻 细胞膜与细胞内脂肪酸 脂肪酸组成 

分 类 号：Q946[生物学—植物学]