PICK-1蛋白在多种肿瘤中的表达及在乳腺癌的临床意义  

The Expression of PICK-1 Protein in Breast Cancer and Its Significance

作　　者：张斌[1] 曹文枫[2] 刘岩雪[2] 张飞[3] 李小莉[4] 林辉[1] 焦振山[4] 曹旭晨[1] 郝希山[1] 

机构地区：[1]乳腺癌防治教育部重点实验室天津市肿瘤防治重点实验室天津医科大学附属肿瘤医院乳腺肿瘤科,天津市300060 [2]乳腺癌防治教育部重点实验室天津市肿瘤防治重点实验室天津医科大学附属肿瘤医院病理科,天津市300060 [3]乳腺癌防治教育部重点实验室天津市肿瘤防治重点实验室天津医科大学附属肿瘤医院中心实验室,天津市300060 [4]天津市长征医院中心实验室

出　　处：《中国肿瘤临床》2009年第7期397-403,共7页Chinese Journal of Clinical Oncology

基　　金：天津市卫生局科技基金项目资助(编号:05KY33)

摘　　要：目的:主要探讨乳腺浸润性癌组织中PICK-1蛋白的表达与该肿瘤临床病理、其他生物学指标以及患者预后的相关性。方法:采用组织芯片免疫组织化学染色方法检测496例乳腺浸润性癌组织中PICK-1、ER、PR、HER2/neu、TlMP-1等蛋白的表达情况及表达间的相关性,以及PICK-1蛋白在不同分子亚型乳腺癌中的表达情况及对预后的影响。结果:PICK-1蛋白在乳腺癌组织中的表达率为27.22%,肿瘤细胞中PICK-1的高表达与淋巴结受累和高组织学分级相关(P均<0.001),且与HER2/neu表达相关(P<0.001),而与雌孕激素受体状态无关(P>0.05),同时该蛋白的表达与TIMP-1表达呈正相关(P=0.002)。PICK-1蛋白表达与患者总生存期缩短相关,分层分析显示PICK-1仅对淋巴结阳性患者的预后有影响,同时对HER2/neu不同状态患者的影响不同,其中对三阴性乳腺癌(HR-HER-)预后影响最大。单因素分析显示乳腺肿瘤直径、组织学分级、淋巴结状态、HR状态、HER2/neu表达、TIMP-1表达、肿瘤细胞表达PICK-1均是有意义的预后指标;但多因素分析显示仅肿瘤直径、组织学分级、淋巴结状态、HER2/neu袁达和PICK-1表达是独立的预后因素。结论:PICK-1蛋白与浸润性乳腺癌的侵袭和转移特性相关,同时有可能作为患者预后不良的指标,尤其是对于三阴性乳腺癌的预后意义更为显著。Objective： To investigate the expression of PICK-1 protein in invasive breast carcinoma and to determine its relationship with the clinicopathologic features, other biological indicators, and prognosis. Methods： We used immunohistochemistry to detect the expressions of PICK-1, estrogen receptor, progesterone receptor, HER2/neu, and TIMP-1 in paraffin-embedded tissue microarrays from 496 cases of invasive breast carcinoma. Results： PICK-1 protein was detected in the cytoplasm of malignant cells in 27.22% cases. High expression of PICK-1 protein within cancer cells was associated with lymph node infiltration and higher grade （P〈0.001） and was positively correlated with HER2/neu expression （P〈0.001）. High expression of PICK-1 protein was also associated with expression of TIMP-1 （P=0.002）. Moreover, high expression of PICK-1 was associated with short overall survival and subsets of positive lymph node. Tumor-derived PICK-1 has different impact on patients with different HER2/neu status, especially for HR-HER-type. Univariate survival analysis showed that tumor size, grade, lymph node status, HR status, HER2/neu expression, TIMP-1 expression, and PICK-1 expression were prognostic factors. Multivariate survival analysis showed that tumor size, grade, lymph node status, HER2/neu expression, and PICK-1 expression were independent prognostic factors. Conclusion： PICK-1 protein is correlated with an invasive and metastatic tumor phenotype and may be used as a prognostic marker of breast carcinoma.

关 键 词：PICK-1 乳腺浸润性癌 组织芯片 预后HER2/neu 

分 类 号：R737.9[医药卫生—肿瘤]