罗格列酮对裸小鼠胃癌移植瘤细胞的抗增生及诱导分化作用  

Antiproliferation and induction differentiation of rosiglitazone in human gastric carcinoma transplanted into nude mice

作　　者：陈方志[1] 朱理辉[1] 胡剑峰[1] 张琍[1] 李国庆[1] 文澜[1] 

机构地区：[1]南华大学附属第二医院消化内科,湖南衡阳421001

出　　处：《肿瘤研究与临床》2007年第6期367-369,共3页Cancer Research and Clinic

摘　　要：目的研究罗格列酮(ROS)对人胃癌MGC-803细胞裸小鼠移植瘤的抗增生和诱导分化作用,初步探讨其可能的机制。方法随机分为未用药组(B组)、全反式维甲酸(ATRA)组(C组)、D_1组(ROS 25mg/kg)、D_2组(ROS 50mg/kg)、D_3组(ROS 100mg/kg)。用药后,观察移植瘤体积变化及计算抑瘤率;检测细胞周期及胃黏蛋白(Mucin 5AC)。结果ROS能缩小瘤体体积,B组与未用药组比较差异有统计学意义(P<0.01),且呈剂量依赖关系,D_1、D_2、D_3组和C组差异无统计学意义(P>0.05);ROS使瘤细胞显示分化细胞的特征:细胞被阻滞于G_0/G_1期,S期细胞减少;Mucin 5AC表达增强。结论ROS有一定的抑瘤作用,呈剂量依赖关系;ROS诱导胃癌移植瘤细胞分化,其机制可能是通过抑制癌细胞从G_1期向S期过渡、降低细胞的增生能力、调控胃癌增生、分化相关基因表达实现的。Objective To investigate the antiproliferation and induction differentiation of human gas- tric carcinoma which human gastric lower-differentiation mucinous carcinoma MGC-803 cells transplanted in- to nude mice by using rosiglitazone(ROS),and to preliminarily explore the mechanism of differentiation. Methods The mice were randomly divided into five groups:model,ATRA,ROS 25 mg·kg^(-1),ROS 50 mg/kg, ROS 100 mg/kg.After that the volumes were measured and inhibition rates were calculated.The cell cycle was detected by FCM.The protein expression level of Mucin SAC was detected by immunohistochemistry. Results The volume of tumor decreased significantly in ROS treatment groups,the differences had statistical significance compared with model group(P<0.01),with the increase of ROS dose,the inhibition rates were al- so increased.The inhibition rate between ROS and ATRA had no significant difference(P>0.05).The xenograft tumors of ROS groups demonstrated the characteristics of differentiation.Xenograft tumor cells were arrested in G_0/G_1 phase,and the cells in S phase decreased significantly,and up-regulated Mucin SAC gene expression.Conclusion ROS could inhibit the growth of tumor,and the effect were dose-dependent with ROS.ROS could induce the differentiation of Xenograft tumor cells of gastric cancer.Its mechanism might be related to the inhibit of transition from G_1 to S phase,degrade the activity of proliferation,regulate the expres- sion of Mucin 5AC.

关 键 词：胃肿瘤 罗格列酮 细胞增生 酶诱导 

分 类 号：R735.2[医药卫生—肿瘤]