The effects of detergents DDM and β-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b_6f complex from spinach chloroplasts  

作　　者：CHEN XiaoBo1,3, ZHAO XiaoHui2, ZHANG JianPing2, LI LiangBi1& KUANG TingYun1 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2 State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sci- ences, Beijing 100080, China 3 Graduate University of Chinese Academy of Sciences, Beijing 100039, China 

出　　处：《Science China(Life Sciences)》2007年第4期505-510,共6页中国科学（生命科学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos.39890390 and 20673144)

摘　　要：The singlet excited state lifetime of the chlorophyll a (Chl a) in cytochrome b6f (Cyt b6f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b6f com-plexes from different sources (~200 and ~600 ps are the two measured results). The present study demonstrated that the singlet excited state lifetime is associated with the detergents n-dodecyl-β-D- maltoside (DDM) and n-octyl-β-D-glucopyranoside (β-OG), but has nothing to do with the different sources of Cyt b6f complexes. Compared with the Cyt b6f dissolved in β-OG, the Cyt b6f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, ~200 ps, of the Chl a in Cyt b6f complex in DDM is closer to the true in vivo.

关 键 词：CYTOCHROME b6f DETERGENT chlorophyll a SINGLET excited state LIFETIME SINGLET oxygen 

分 类 号：Q946[生物学—植物学]