不同中西蜂种的RAPD分析  

Studyon RAPD Analysis of the Genetic Diverity among Different Race of Bees

作　　者：张学锋[1,2] 孙亮先[3] 谢宪兵[1,3] 郭冬生[4] 曾志将[1] 罗岳雄[2] 黄文忠[2] 陈华生[2] 

机构地区：[1]江西农业大学蜜蜂研究所,江西南昌330045 [2]广东省昆虫研究所,广东广州512060 [3]福建泉州师范学院,福建泉州362000 [4]江西宜春学院,江西宜春336000

出　　处：《蜜蜂杂志》2007年第S1期52-56,共5页Journal of Bee

摘　　要：利用随机扩增多态性DNA(RAPD)技术,对国内目前主要饲养的不同蜂种:蜜胶1号(杂交蜂)、美国意蜂、原意、东北黑蜂、卡尼鄂拉蜂、喀尔巴阡蜂、高加索蜂、澳意、江西南昌本意、浙江江山本意、广州本意和广州中蜂的遗传多样性进行了分析和比较。用14个随机引物对这12种不同蜂种进行了随机扩增,12个随机引物共扩增出86条谱带,其中51条谱带具有多态性,占59.4%,每个引物可扩增出2—7条多态性谱带,平均4条。根据Nei氏公式计算出12种蜜蜂的相对遗传距离,并进行聚类分析。通过比较分析,不同蜂种之间的遗传距离分布范围在0.185 4—1.268 5。遗传距离计算和聚类结果与实际基本相符。The genetic diversity was studied by the method of RAPD among 12 races of bees in China- Bee I of highly producing honey and propolis,American_Italian bee,Apis mellifera L,Apis mellifera ssp,Apis mellifera carnicc,Apis mel- lifera carpatica,Apis mellifera carpatica,Australia_Italian bee,Apis mellifera L from NanChang of JiangXi,Apis mellifera L from JiangShan of ZheJiang,Apis mellifera L from GuangZhou,Apis cerana cerana from GuangZhou.Fourteen random primers were used in the Random Amplified Poly- morphic DNA(RAPD) analysis of the 12 kinds of bees.Among the fourteen random primers,twelve primers had obviously special bands in those bees.It was polymorphic for 51 of 86 bands produced by the random primers.The polymorphic percent was 59.4%.Each primer could amplify 2 to 7 poly- morphic bands,with an average of 4 bands.According to Nei' S formula,the relative genetic distance was counted. A phylogenetic tree was constructed by the UPGMA program based on the genetic distance among the 12 kinds of bees. The genetic distance among bees varied from 0,074 to 0,667,the results matched with the actuality of those bees.

关 键 词：蜜蜂 RAPD 遗传距离 

分 类 号：S892[农业科学—特种经济动物饲养]