造血与淋巴组织肿瘤患者红细胞补体受体1分子数量及其基因组密度多态性测定  

The number of E-CR1 and the red cell CR1 genomic density polymorphism in patients with hematologic and lymphoid neoplasms

作　　者：张乃红[1] 冯玫[1] 朱镭[2] 刘洁[1] 高鸿鹏[1] 李为民[1] 乔振华[2] 

机构地区：[1]山西省人民医院血液科,太原030012 [2]山西医科大学第二医院血液科

出　　处：《白血病．淋巴瘤》2007年第2期85-87,共3页Journal of Leukemia & Lymphoma

摘　　要：目的检测造血与淋巴组织肿瘤患者红细胞补体受体1(CR1)分子数量及其基因组密度多态性的变化。方法采集静脉血,用FITC标记的鼠抗人Cd_(35)单抗标记红细胞,应用流式细胞仪测定了24例造血与淋巴组织肿瘤患者、21例健康对照红细胞CR1分子的数量。采用PCR和HindⅢ酶切技术测定红细胞CR1密度相关基因组的多态性。结果造血与淋巴组织肿瘤患者红细胞CR1分子数量平均值为(80.7±13.3)个/红细胞,健康对照的红细胞CR1分子数量平均值为(105.5±8.8)个/红细胞。造血与淋巴组织肿瘤患者与健康对照相比,其红细胞平均CR1分子数量减少,差异有统计学意义(t=7.234,P<0.0001)。造血与淋巴组织肿瘤患者较健康人HH型所占比例降低,HL型所占比例升高,但差异无统计学意义。结论遗传基因的变化可能引起造血与淋巴组织肿瘤患者红细胞CR1分子数量的改变。Objective To detect the number of E-CR1 and study the change regularity of E-CR1 genomic density polymorphism of patients with hematologic and lymphoid neoplasms and that of normal con- trois.Methods Red blood cells from venous blood are labeled by mouse anti-human CD_(35)-FITC monoclonal antibody.Using flow cytometry,we determine the number of ECR1 of 24 patients with hematologic and lym- phoid neoplasms and that of 21 normal controls.Polymerase chain reaction (PCR) and HindⅢrestriction en- zyme digestion were used to detect the change of E-CR1 genomic density polymorphism.Results The mean value of the number of CR1 on each RBC of 24 patients is (80.7±13.3) and that of the normal controls is (105.5±8.8).Comparing with the controls,ECR1 of the patients with hematologic and lymphoid neoplasms are decreased significantly (t=7.234,P<0.0001).The E-CR1 genomic density polymorphism distribution state was type HH 76.2 %,HL 14.3%,LL 9.5 %,and that of the patients was HH 58.3 %,HL 29.2 %,LL 12.5 %. The spot mutation rate of ECR1 gene in patients with hematologic and lymphoid neoplasms was higher than that in normal people but has no significant deviation.Conclusion The spot mutation of E-CR1 gene in pa- tients with hematologic and lymphoid neoplasms may be related to the change of the number of E-CR1.

关 键 词：血液肿瘤 受体 补体3b 

分 类 号：R733[医药卫生—肿瘤]