癌蛋白iASPP对小鼠骨髓细胞增生促进作用研究  

iASPP oncoprotein potentates the proliferation peculiarity of cells derived from murine bone marrow

作　　者：邢海燕[1] 张新伟[2] 刘向荣[1] 唐克晶[1] 田征[1] 刁世勇[1] 饶青[1] 王敏[1] 王建祥[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所血液病医院,实验血液学国家重点实验室,天津300020 [2]天津市肿瘤医院

出　　处：《白血病．淋巴瘤》2007年第5期324-326,共3页Journal of Leukemia & Lymphoma

基　　金：国家自然科学基金(30470750);天津市自然科学基金(043607311)

摘　　要：目的研究 iASPP(一个新发现的 p53家族抑制性成员)在小鼠骨髓细胞中过表达对细胞增生作用的影响。方法构建反转录病毒载体,通过基因转导方法,使小鼠骨髓细胞过表达人类 iASPP或(和)突变的 N-Ras,采用集落形成实验检测细胞体外增生能力的变化。结果转导 iASPP 的小鼠骨髓细胞与转导空载体对照组相比集落形成能力显著增强(P<0.01),转导突变的 N-Ras 也能提高小鼠骨髓细胞的体外增生能力,二者差异无统计学意义(P>0.05),但 iASPP 联合突变的 N-Ras 可使集落的数量显著多于单基因组(P<0.05)及空载体对照组(P<0.01),而且集落体积增大。结论癌蛋白 iASPP 可以显著提高小鼠骨髓细胞的体外增生能力,并且与突变的 N-Ras 具有协同作用,因而 iASPP 有望成为研究肿瘤及白血病治疗的新靶点。Objective To explore the effects of iASPP(inhibitory member of ASPP of p53 family) overexpression on murine bone marrow cells(BMC)proliferation.Methods BALB/c murine BMC were infect- ed with retroviral vector expressing human iASPP or/and mutant N-Ras.The proliferation in vitro of transduced BMC were detected by colony forming assay.Results The colony forming frequency of iASPP-murine BMC was higher than that of empty vector transduced BMC(P<0.01),so was the N-Ras transduced BMC.When i- ASPP and N-Ras were cotransduced into BMC,the colonies were more and bigger(P<0.05).Conclusion iASPP oncoprotein could enhance the proliferation potentiality of murine BMC in vitro,and it has synergistic effect with mutant N-Ras.So iASPP could be exploited as new therapeutic target of tumor and leukemia.

关 键 词：癌基因蛋白质类 IASPP 转导 遗传 反转录病毒 

分 类 号：R73-3[医药卫生—肿瘤]