胃癌中p14、p15、p16基因微卫星不稳定性的研究  

Study on microsatemte instability of p14,p15,p16 gene in gastric carcinoma

作　　者：庞瑾昱[1] 杨宣琴[2] 李素红[2] 李丽[2] 王全红[2] 

机构地区：[1]山西医科大学病理教研室,太原030001 [2]山西省肿瘤医院病理科

出　　处：《肿瘤研究与临床》2007年第8期512-515,共4页Cancer Research and Clinic

摘　　要：目的对照检测伴有正常及高级别上皮内瘤变的胃腺癌组织中定位于染色体9p21区D9S166、D9S171、D9S941、D9S942和IFNA的微卫星不稳定性(MSI),探讨p14、p15和p16基因与胃癌发生发展的关系。方法选择55例伴有正常及高级别上皮内瘤变的胃腺癌标本,应用手工显微切割、聚合酶链反应、高压凝胶电泳、硝酸银染色等技术,对照分析正常、高级别上皮内瘤变和胃腺癌组织中9p21上的5个微卫星位点的MSI变化。结果胃癌组织MSI总发生率为27%(64/233),高级别上皮内瘤变MSI总发生率为18%(42/233),正常组织中未发生MSI。胃癌组织中的MSI发生率显著高于高级别上皮内瘤变(P<0.05)。在胃癌组织中,5个微卫星位点的MSI发生率依次为D9S171(45%)、IFNA (41%)、D9S941(22%)、D9S166(16%)、D9S942(15%);高级别上皮内瘤变组织中,依次为IFNA (22%)、D9S171(21%)、D9S941(18%)、D9S166(14%)、D9S942(11%)。在胃癌组织中D9S171位点的MSI发生率为45%,在高级别上皮内瘤变的MSI发生率为21%。两者差异有统计学意义(P<0.05)。结论胃癌中染色体9p21区发生有高频率的MSI,MSI可能为胃癌发生过程中的早期分子事件,并且在胃癌发展过程中持续发挥作用。p14、p15和p16基因可能与胃癌的发生发展相关。Objective To detect the microsatellite instability (MSI) of D9S166,D9S171,D9S941, D9S942 and IFNA located at chromosome 9p21 in gastric carcinoma with high-grade intraepithelial neoplasia and normal tissue,and investigate the correlation of p14,p15,p16 gene and the gastric carcinogenesis. Methods 55 cases of gastric carcinoma with high-grade intraepithelial neoplasia and normal tissue were se- lected to detect MSI by microdissection,polymerase chain reaction,denaturing polyaerylamide gel elec- trophoresis and silver nitrate staining technology of five microsatellite markers on 9p21.Results In the infor- mative cases,total ratio of MSI in gastric carcinoma was 27 % (64/233) and it was 18% (42/233) in high- grade intraepithelial neoplasia.The ratio of MSI was significantly higher in gastric carcinoma than that in high-grade intraepithelial neoplasia (P<0.05).In gastric carcinoma the ratio of MSI was:D9S171(45%),IF- NA(41%),D9S941(22%),D9S166(16%) and D9S942(15%) respectively.In high-grade intraepithelial neoplasia,MSI ratio was as follows:IFNA(22%),D9S171(21%),D9S941(18%),D9S166(14%) and D9S942 (11%).The ratio of MSI in the microsatellite site D9S171 of gastric carcinoma and high-grade in- traepithelial neoplasia were 45% and 21% respectively,there was significant difference between them.(P<0.05).Conclusion The ratio of MSI is higher on chromosome 9p21 in gastric carcinoma.MSI probably is an early event during gastric carcinogenesis,p14,p15,p16 gene are probably correlated with gastric carcinogenesis.

关 键 词：胃肿瘤 腺癌 高级别上皮内瘤变 微卫星不稳定性 基因 p14 p15 p16 

分 类 号：R735.2[医药卫生—肿瘤]