中期因子mRNA在乳腺癌中的表达及其意义  

Expressions of midkine mRNA(MKmRNA)in breast carcinoma and its significance

作　　者：平金良[1] 戴利成[2] 章步文[1] 马志红[2] 赵华[1] 倪灿荣[3] 

机构地区：[1]浙江省湖州市中心医院病理科,313000 [2]浙江省湖州市中心医院中心实验室,313000 [3]第二军医大学附属长海医院病理科

出　　处：《肿瘤研究与临床》2007年第5期308-310,共3页Cancer Research and Clinic

摘　　要：目的研究中期因子(midkine,MK)mRNA在乳腺癌组织中的表达及其意义。方法应用原位杂交技术方法,检测56例乳腺癌组织及其配对的癌旁乳腺组织MK mRNA表达,并结合临床、病理及随访资料进行分析。结果56例乳腺癌组织中MK mRNA阳性表达率为60.7%,阳性信号定位在瘤细胞胞质内,癌旁乳腺组织不表达或仅微弱阳性。乳腺癌TNM分期Ⅰ、Ⅱ期和淋巴结未转移组MK mRNA阳性表达率明显低于Ⅲ、Ⅳ期和淋巴结转移组(P=0.045,P=0.027),生存曲线分析表明MK mRNA阳性表达组患者的生存率明显低于阴性组(P=0.039),与乳腺癌年龄、月经状况、肿块大小、组织学分级均无相关性(P>0.05)。结论乳腺癌组织中MK mRNA呈高表达,检测MK表达可作为判定乳腺癌恶性度、浸润转移等生物学行为的有价值的参考指标。Objective To investigate the expression of midkine(MK mRNA)and its significance in breast carcinoma.Methods The expression of MK mRNA was studied in situ hybridization in 56 patients of breast carcinoma tissue and their paired breast tissue.Results The positive rate of MK mRNA in the breast carcinoma tissue was 60.7%.The breast carcinoma tissues showed enhanced positive reaction cytoplasm to the MK mRNA and their paired breast tissues showed negative or weakly positive.The positive rate of MK mRNA was significantly lower in the cases of TNMⅠ,Ⅱstage and without lymph node metastasis than that of TNMⅢ,Ⅳstage and lymph node metastasis(P=0.045,P=0.027).The patients with positive MK mRNA expression had a significantly shorter survival than those with negative MK mRNA expression(P=0.039), whereas no correlations with age,menopausal status,tumor size,histologic grade(P>0.05).Conclusion MK mRNA overexpresses in the breast cancer tissues.It might be considered to be a reference indicator for deter- mining the angiogenesis and invasion of breast carcinoma.

关 键 词：乳腺肿瘤 生长物质 原位杂交 转移 预后 

分 类 号：R737.9[医药卫生—肿瘤]