Direct electrochemistry behavior of Cytochrome c on silicon dioxide nanoparticles-modified electrode  

作　　者：ZHU Lin SUN DongMei LU TianHong CAI ChenXin LIU ChangPeng XING Wei 

机构地区：[1]Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [2]Graduate University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Chemistry and Environmental Science,Nanjing Normal University,Nanjing 210097,China

出　　处：《Science China Chemistry》2007年第3期304-307,共4页中国科学（化学英文版）

基　　金：Supported by the Fundamental Research Program 973 Program (Grant No. G2000026408);High Technology Research Program of Science and Technology Ministry of China 863 Program (Grant Nos. 2001AA323060, 2003AA517062);the National Natural Science Foundation of China (Grant No. 20373062);Key Pro-ject (Grant No.20433060);Research Foundation of State Key Laboratory of Physical Chemistry of Solid Surface (Grant No. 200407)

摘　　要：A newfangled direct electrochemistry behavior of Cytochrome c (Cyt c) was found on glassy carbon (GC) electrode modified with the silicon dioxide (SiO2) nanoparticles by physical adsorption. A pair of stable and well-defined redox peaks of Cyt c′ quasi-reversible electrochemical reaction were obtained with a heterogeneous electron transfer rate constant of 1.66×10-3 cm/s and a formal potential of 0.069 V (vs. Ag/AgCl) (0.263 V versus NHE) in 0.1 mol/L pH 6.8 PBS. Both the size and the amount of SiO2 nanoparticles could influence the electron transfer between Cyt c and the electrode. Electrostatic interaction which is between the negative nanoparticle surface and positively charged amino acid residues on the Cyt c surface is of importance for the stability and reproducibility toward the direct electron transfer of Cyt c. It is suggested that the modification of SiO2 nanoparticles proposes a novel approach to realize the direct electrochemistry of proteins.A newfangled direct electrochemistry behavior of Cytochrome c (Cyt c) was found on glassy carbon (GC) electrode modified with the silicon dioxide (SiO2) nanoparticles by physical adsorption. A pair of stable and well-defined redox peaks of Cyt c′ quasi-reversible electrochemical reaction were obtained with a heterogeneous electron transfer rate constant of 1.66×10?3 cm/s and a formal potential of 0.069 V (vs. Ag/AgCl) (0.263 V versus NHE) in 0.1 mol/L pH 6.8 PBS. Both the size and the amount of SiO2 nanoparticles could influence the electron transfer between Cyt c and the electrode. Electrostatic interaction which is between the negative nanoparticle surface and positively charged amino acid residues on the Cyt c surface is of importance for the stability and reproducibility toward the direct electron transfer of Cyt c. It is suggested that the modification of SiO2 nanoparticles proposes a novel approach to realize the direct electrochemistry of proteins.

关 键 词：CYTOCHROME c silicon dioxide NANOPARTICLE DIRECT ELECTRON TRANSFER 

分 类 号：O657.1[理学—分析化学]