In situ synthesis of DNA micro-arrays using typography technique  

作　　者：TANG JianXin 1,2 ,HE NongYue 1,2,3 &LI Song 1 1State Key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China 2Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province,Hunan University of Tech- nology,Zhuzhou 412008,China 3Zhongshan Huoju Polytechnic,Zhongshan 528436,China 

出　　处：《Science China Chemistry》2007年第1期75-83,共9页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China(Grant Nos.60571032,60571001,90606027 and 60121101);National Hi-Tech Research andDevelopment Program of China(Grant No.2006AA03Z357);Natural ScienceFoundation of Hunan Province(Grant Nos.04jj40023 and 06JJ4012);the NaturalScience Foundation of Guangdong Province(Grant No.04008782)

摘　　要：A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide,which has the celerity,high spatial resolution,lower cost,reliable operation,and high synthetic efficiency.The principle and process of the typography technique for fabricating gene-chips have been described in detail.A suit of poly(terafluoroethylene)devices for synthesizing oligonucleotide arrays were designed and prepared,and the fiber tubes with a number of nano-or micron-channels were em- ployed.The oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography technique.The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals.It was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms(SNP)and to detect mutations.A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide, which has the celerity, high spatial resolution, lower cost, reliable operation, and high synthetic efficiency. The principle and process of the typography technique for fabricating gene-chips have been described in detail. A suit of poly(terafluoroethylene) devices for synthesizing oligonucleotide arrays were designed and prepared, and the fiber tubes with a number of nano-or micron-channels were employed. The oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography technique. The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals. It was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms (SNP) and to detect mutations.

关 键 词：TYPOGRAPHY OLIGONUCLEOTIDE in SITU synthesis DNA microarrays 

分 类 号：Q78[生物学—分子生物学]