SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin  

作　　者：ZHANG ZhaoXia ZHAO HongWei ZHU HongPing GE Min WANG WenFeng YAO SiDe LI WenXin 

机构地区：[1]Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China [2]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China Chemistry》2007年第1期84-90,共7页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China(Grant Nos.20373086 and 10574134);the research program of the Chinese Academy ofSciences

摘　　要：The photooxidation damage of lysozyme under 315-375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).Indica- tions showed that the mechanisms and products of oxidative damage were relative to the concentra- tion of riboflavin,the time of irradiation and the ambience.The type I process was examined in a nitrogen saturated solution,whereas both type I and type II were observed in an aerobic atmosphere and type II was the dominant process.The study also suggested that antioxidants,such as melatonin, can reduce the damage of lysozyme effectively.The photooxidation damage of lysozyme under 315–375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Indications showed that the mechanisms and products of oxidative damage were relative to the concentration of riboflavin, the time of irradiation and the ambience. The type I process was examined in a nitrogen saturated solution, whereas both type I and type II were observed in an aerobic atmosphere and type II was the dominant process. The study also suggested that antioxidants, such as melatonin, can reduce the damage of lysozyme effectively.

关 键 词：LYSOZYME RIBOFLAVIN PHOTOOXIDATION SDS-PAGE ANTIOXIDANT 

分 类 号：Q563.2[生物学—生物化学]