A rhizobia strain isolated from root nodule of gymno-sperm Podocarpus macrophyllus  

作　　者：HUANG BaoLing, LU ChengQun, WU Bo & FAN LiQin 1 Forestry College, Guangxi University, Nanning 530004, China 2 Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning 530004, China 

出　　处：《Science China(Life Sciences)》2007年第2期228-233,共6页中国科学（生命科学英文版）

基　　金：the National Natural Science Foundation of China(GrantNo.30170033)

摘　　要：Symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. Symbiotic nodulation of gymnosperm Podocarpus macro-phyllus and rhizobia has never been reported. In this study, 11 endophytic bacteria strains were isolated from root nodules of P. macrophyllus and its variation P. macrophyllus var. maki. The plant infection tests on these strains indicated that the isolated strains could be nodulated on P. macrophyllus plants, and weak nitrogenase activity of nodules was found in acetylene reduction method. According to the physiological and biochemical characteristics of the 11 strains, GXLO 02 was selected as the representative strain. 16S rDNA full-length sequence analysis of GXLO 02 confirmed that the representative strain GXLO 02 belongs to Rhizobium sp.Symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. Symbiotic nodulation of gymnosperm Podocarpus macro-phyllus and rhizobia has never been reported. In this study, 11 endophytic bacteria strains were isolated from root nodules of P. macrophyllus and its variation P. macrophyllus var. maki. The plant infection tests on these strains indicated that the isolated strains could be nodulated on P. macrophyllus plants, and weak nitrogenase activity of nodules was found in acetylene reduction method. According to the physiological and biochemical characteristics of the 11 strains, GXLO 02 was selected as the representative strain. 16S rDNA full-length sequence analysis of GXLO 02 confirmed that the representative strain GXLO 02 belongs to Rhizobium sp.

关 键 词：PODOCARPUS macrophyllus P. macrophyllus VAR. maki RHIZOBIA PHYSIOLOGICAL and BIOCHEMICAL characteristics 16S rDNA sequence 

分 类 号：S154.3[农业科学—土壤学]