Presence of donor-and-recipient-derived DNA microchimerism in the cell-free blood samples of renal transplantation recipients associates with the acceptance of transplanted kidneys  

肾移植受者无细胞血样中出现供受者来源的DNA微嵌合体(英文)

作　　者：Yao-Wen Fu Wei-Gang Wang Hong-Lan Zhou Lu Cai 

机构地区：[1]Renal Transplantation Center of Jilin Province,and Department of Urology,the China-Japan Union Hospital,Changchun 130033,China [2]Department of Hematology and Oncology,the First Teaching Hospital,Jilin University,Changchun 130021,China [3]Departments of Medicine,Radiation Oncology,and Pharmacology and Toxicology,the University of Louisville,Louisville,Kentucky 40202,USA

出　　处：《Asian Journal of Andrology》2006年第4期477-482,517,共6页亚洲男性学杂志（英文版）

摘　　要：Aim:To examine whether the existence of the donor-and recipient-derived DNA chimerism in recipient's plasma can be a predictive marker for the status of transplanted organ.Methods:One hundred and twenty-six female patients who had been transplanted with male kidneys were enrolled in the present study.In these female recipients,the SRY_Ⅰ, DYZ_I^(Ⅰst)and DYZ_Ⅰ^(2nd)genes on the Y chromosome from the plasma were prospectively examined using reverse tran- scription polymerase chain reaction(RT-PCR).Results:SRY,DYZ_Ⅰ^(Ⅰst)and DYZ_Ⅰ^(2nd)sequences were detected in the cell-free blood(plasma)of 97(77%)of 126 female patients with male kidney.The average time that the transplanted kidneys functioned was 8.7 years and 5.4 years among microchimerism-positive and microchimerism-negative recipients,respectively.The frequency of the patients who had acute rejection after renal transplantation was ap- proximately 10% and 28% in microchimerism-positive and microchimerism-negative recipients,respectively.Serum creatinine levels in microchimerism-positive patients were significantly lower than those in microchimerism-negative patients.Conclusion:These results suggest that plasma DNA microchimerism present in certain patients following renal transplantation and measurement of plasma DNA microchimerism using quantitative RT-PCR might be a useful predictor for the acceptance of transplanted kidneys.(Asian J Androl 2006 Jul;8:477-482)Aim:To examine whether the existence of the donor-and recipient-derived DNA chimerism in recipient's plasma can be a predictive marker for the status of transplanted organ.Methods:One hundred and twenty-six female patients who had been transplanted with male kidneys were enrolled in the present study.In these female recipients,the SRY_Ⅰ, DYZ_I^(Ⅰst)and DYZ_Ⅰ^(2nd)genes on the Y chromosome from the plasma were prospectively examined using reverse tran- scription polymerase chain reaction(RT-PCR).Results:SRY,DYZ_Ⅰ^(Ⅰst)and DYZ_Ⅰ^(2nd)sequences were detected in the cell-free blood(plasma)of 97(77%)of 126 female patients with male kidney.The average time that the transplanted kidneys functioned was 8.7 years and 5.4 years among microchimerism-positive and microchimerism-negative recipients,respectively.The frequency of the patients who had acute rejection after renal transplantation was ap- proximately 10% and 28% in microchimerism-positive and microchimerism-negative recipients,respectively.Serum creatinine levels in microchimerism-positive patients were significantly lower than those in microchimerism-negative patients.Conclusion:These results suggest that plasma DNA microchimerism present in certain patients following renal transplantation and measurement of plasma DNA microchimerism using quantitative RT-PCR might be a useful predictor for the acceptance of transplanted kidneys.(Asian J Androl 2006 Jul;8:477-482)

关 键 词：renal transplantation donor-and-recipient-derived DNA microchimerism IMMUNOTOLERANCE renal rejection 

分 类 号：R699[医药卫生—泌尿科学]