不同浓度自体骨髓基质细胞移植修复Beagle犬Ⅱ°根分叉病变的研究  

A Study on the Repair of Canine Experimental Class n Furcation Defects by Bone Marrow Stromal Cells with Different Seeding Density

作　　者：闫福华[1] 陈欣戬[1] 詹暶[1] 林敏魁[1] 赵欣[1] 

机构地区：[1]福建医科大学口腔医学院,350002

出　　处：《组织工程与重建外科杂志》2005年第6期312-315,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：福建省科技重大项目(2001Z021)福建医科大学学科带头人培养计划(KQYY2003)

摘　　要：目的比较不同浓度自体骨髓基质细胞修复牙周组织缺损的能力。方法在6只一岁半的Beagle犬的下颌牙制备慢性Ⅱ°根分叉病变模型,在GTR的基础上,用不同浓度的BMSCs(5×105／ml组,5×106／ml组,5×107／ml组)移植治疗,12周后作病理切片,HE染色观察并测量牙周组织再生情况。结果GTR组的新生牙骨质长度和新生牙槽骨面积的百分比分别为:51．5±5．6和27．1±7．7;5×105／ml组为:84．8±8．9和30．6±7．7;5×106／ml组为:91．8±5．2和68．3±11．4;5×107／ml组为:88．8±7．2和78．5±12．7。细胞组的新生牙骨质量与对照组相比差异有显著性(p<0．01),但各细胞组间相互比较无差异显著性;5×106／ml组和5×107／ml组的新生牙槽骨量与5×105／m／组和对照组相比差异有显著性(p<0．01),但前两组间和后两组间比较则差异无显著性。结论骨髓基质细胞促进牙周组织再生的作用与细胞的接种密度有关,高浓度细胞能有效促进牙周组织再生。Objective To compare the difference in promoting periodontal regeneration of initial cell seeding density in canine experimental class II furcation defects . Methods GTR combination with the expanded BMSCs which were seeded in collagen membrance at initial concentrations of 5×105 cells/ml,5×106 cells /ml or 5×107 cells /ml, and auto-transplanted into experimental class II furcation defects, underneath a Bio-Gide membrane. Bio-Gide membrane alone was implanted into the defects as a control. Periodontal regeneration was evaluated by histologic and histometric analyses 12 weeks after transplantation. Results The percentage of new cementum length in the 5×105 cells/ml,5×106 cells /ml and 5×107 cells /ml groups were significantly higher than in the control group (p<0.01), and there was no significantly difference in the three cell groups. The percentage of new alveolar bone area in the 5×106 cells /ml and 5×107 cells /ml groups were significantly higher than in the control and 5×105 cells/ml group (p<0.01), and there was no significantly difference between the first two groups, and neither was the later. Conclusions This study suggested that the effect of auto-transplanted BMSCs promoting periodontal healing has cell density-dependent under certain conditions, and the higher concentrations(5×106 cells /ml and 5×107 cells /ml) can effectively promote periodontal regeneration.

关 键 词：牙周组织再生 骨髓基质细胞 种子细胞 细胞密度 支架材料 

分 类 号：R783.3[医药卫生—口腔医学]