药用蛋白冷冻干燥过程中变性及相应的保护措施  

A review on the denaturation mechanism of protein and its corresponding protection measures

作　　者：耿旭[1] 李骏[1] 卞慧芳[1] 吴自荣[1] 

机构地区：[1]华东师范大学生命科学学院分子生物学实验室,上海200062

出　　处：《云南大学学报（自然科学版）》2004年第S2期136-139,共4页Journal of Yunnan University(Natural Sciences Edition)

摘　　要：冷冻干燥是制备蛋白质药物常用方法.详细综述了冷冻干燥过程中导致蛋白质变性机理的种类、特征、原理;介绍了主要的保护措施即添加保护剂,并阐述了保护剂的作用机理:优先化作用、玻璃态假说和水代替假说.The method most commonly used for pharmaceutical protein products is lyophilization. The classification, characters, and mechanism of denaturation protein on Freeze-dry process are thorough discussed. To protect a protein from freezing and dehydration, a protein stabilizers may be used. There are primary three mechamism of protein stabilization during lyophilization, which are the preferential interaction, the fonnation of an amorphous glass and the water replacement.

关 键 词：冷冻干燥 变性机理 保护机理 保护剂 蛋白质变性 

分 类 号：TQ464.7[化学工程—制药化工]