知母皂甙元对大鼠脑内总M及M_1受体的影响  

The Effect of ZMS on Brain Total M and M_1 Receptor Densities in Young and Aged Rats

作　　者：张乃钲[1] 孙启祥[1] 胡雅儿[1] 夏宗勤[1] 

机构地区：[1]上海第二医科大学细胞调控研究室,上海200025

出　　处：《上海交通大学学报（医学版）》2001年第6期481-483,共3页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金资助项目 (3932 0 0 0 4 )

摘　　要：目的观察知母皂甙元对青年和老年大鼠脑M及M1受体的不同影响。　方法以对M1受体有相对选择性的哌仑西平为非标记竞争剂 ,3H -QNB为非选择性标记配基 ,建立M和M1受体的放射配基竞争结合分析方法 ,观察知母皂甙元对青年大鼠和老年大鼠脑M和M1受体的影响。　结果与青年大鼠相比 ,老年大鼠脑M及M1受体减少 ,知母皂甙元能上调M和M1受体 ;对正常青年大鼠脑M及M1受体的密度 ,知母皂甙元没有影响。结论知母皂甙元对下降的M及M1受体密度有明显的上调作用 ,但不超过正常值 ,对正常水平的M及M1受体密度 。Objective To explore whether the effects of a sapogenin from Zhimu (ZMS) on cerebral M and M 1 receptor densities are different in young rats and aged rats. Methods A dual site competitive binding technique was established,using unlabelled pirenzipine as the selective competitive agent and 3H-QNB as the nonselective radioligand. This technique was used to observe the total cerebral M and M 1 receptor densities in aged and young SD rats, treated or untreated with ZMS. ResultsIn aged rats, the densities of total cerebral M and M 1 receptors were both significantly decreased. Oral administration of ZMS for three months up-regulated the total cerebral M and M 1 receptors in aged rats, making the density approaching normal, but similar administration of ZMS has no effect in young rats. Conclusion ZMS can only restore the decreased total cerebral M and M 1 receptor densities, making them approaching normal, but cannot raise the normal densities to a higher level.

关 键 词：知母皂甙元 M胆碱受体 M1受体 放射配基受体竞争结合分析 

分 类 号：R749.161[医药卫生—神经病学与精神病学]