Determination of estradiol metabolites in human liver microsome by high performance liquid chromatography-electrochemistry detector  

高效液相色谱-电化学检测器法测定人肝微粒体中雌二醇的代谢产物(英文)

作　　者：程泽能[1] 黄松林[1] 谭志荣[1] 王伟[1] 周宏灏[1] 

机构地区：[1]湖南医科大学基础与临床药理学研究所

出　　处：《Acta Pharmacologica Sinica》2001年第4期83-88,共6页中国药理学报（英文版）

基　　金：Project supported by China Medical Board 92-568 and 99-697.

摘　　要：AIM: To constitute a method to determine the estradiol metabolites in human liver microsome in low concentration of estradiol. METHODS: Use high performance liquid chromatography after solvent extraction, evaporation, and reconstitution to separate the metabolites and use a electrochemistry detector to detect the metabolites. RESULTS: With a mobile phase of acetic acid buffer-acetonitrile (50:50, v/v, pH 4.5) at flow rate of 1.0 mL/min and a potential of +0.7 V vs Ag/AgCl, all six composition were well separated and satisfactorily detected. There are E3, 16α-OHE1, 2-OHE2, E1; and two unidentified composition. The minimum detectable amount is about 100 pg on column. This method is sensitive enough to detect E1 in a substrate concentration of 1 μmol/L. CONCLUSION: The method can be used to study the metabolism mechanism of estradiol in liver microsome .目的:建立雌二醇在人肝微粒体中代谢产物的测定方法,研究低浓度雌二醇在人肝微粒体中的代谢机制。方法:有机溶剂提取、蒸发、重溶的方法处理样品,高效液相色谱法分离组分,电化学检测器测定含量。结果:流动相为醋酸缓冲液-乙腈(50:50,v/v,pH4.5,电压为+0.7V vs Ag/AgCl时,在C18柱上分离较好。最低检测量为100pg。结论:本法可用于测定底物浓度在1-100μmol/L时,人肝微粒体中的代谢产物。

关 键 词：high pressure liquid chromatography liver microsome ESTRADIOL 

分 类 号：R96[医药卫生—药理学]