Effects of calcitriol and its analogues on interaction of MCP-1 and monocyte-derived dendritic cells in vitro  

骨化三醇及其类似物对MCP-1与单核细胞衍生的树突状细胞间相互作用的影响(英文)

作　　者：朱可建[1] 沈倩云[2] 郑敏[1] Ulrich MROWIETZ 

机构地区：[1]浙江大学医学院附属第二医院皮肤科,杭州中国310009 [2]浙江大学医学院附属第一医院外科,杭州中国310003 [3]基尔大学皮肤病医院

出　　处：《Acta Pharmacologica Sinica》2001年第1期64-68,共5页中国药理学报（英文版）

摘　　要：AIM: To study the effect of calcitriol [1,25(OH)2D3] and its analogues on the interaction of monocyte chemoat-tractant protein-1 (MCP-1) and in vitro generated mono-cyte-derived dendritic cells (MoDC). METHODS: MoDC were obtained by differentiating monocytes in exposure to GM-CSF and IL-4 for 5 d. mRNA expression of MCP-1 and its receptors were analyzed by RT-PCR, and protein production of MCP-1 by ELISA and migratory ability of MoDC in response to MCP-1 by a micromul-tiwell chemotaxis chamber assay. RESULTS: MoDC can express MCP-1 mRNA, and secret a low level of MCP-1 protein and has the ability to migrate to MCP-1 in corresponding to its expression of MCP-1 receptors. Calcitriol and its analogues with the same affinity to vitamin D receptor up-regulated the gene expression of both MCP-1 and its receptors, enhanced MCP-1 protein production and promoted the migratory ability of MoDC to MCP-1. CONCLUSION: The interaction of DC and MCP-1 found in this study may suggest a possible auto-regulatory role between DC and MCP-1 and the modula-tory effect of calcitriol and its analogues on DC and MCP-1 might provide an understanding of their positive role in tumors.目的:研究骨化三醇及其类似物对单核细胞趋化蛋白-1(MCP-1)和单核细胞衍生的树突状细胞(MoDC)两者之间相互作用的影响。方法:用GM-CSF和IL-4使单核细胞经体外培养5天后分化为MoDC。用RT-PCR分析MCP-1及其受体的mRNA表达,ELISA测定蛋白质水平。用微孔化学趋化板检测MoDC对MCR-1的游走功能。结果:MoDC能表达MCP-1 mRNA,分泌低水平MCP-1蛋白,并表达MCP-1受体从而具有向MCP-1游走的功能。骨化三醇及其类似物卡泊三醇和他卡西醇对维生素D受体具有相同亲和力,它们均能上调MoDC的MCP-1和MCP-1受体的表达,增加MCR-1蛋白质水平并促进MoDC对MCP-1的游走功能。结论:树突状细胞(DC)与MCP-1间可能存在自调节作用。骨化三醇及其类似物对DC和MCP-1的调节可使其在肿瘤治疗中起积极作用。

关 键 词：dendritic cells monocyte chemoattractant protein-1 CALCITRIOL CALCIPOTRIOL TACALCITOL 

分 类 号：R96[医药卫生—药理学]