Gene-CYP11B2 expression in rat liver in hepatic fibrogenesis induced by CCl_4  

作　　者：李旭 杨希山 吴平生 孟莹 李淑梅 赖文岩 

出　　处：《Chinese Medical Journal》2001年第1期64-68,共5页中华医学杂志（英文版）

基　　金：theNationalNaturalScienceFoundationofChina (No 39870 331)

摘　　要：identify aldosterone synthase gene CYP11B2 mRNA expression in normal and fibrotic liver in rats and evaluate the curative effect of antisterone Methods 160 Wistar rats weighing about 250?g were divided into 4 groups In the model group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week In the antisterone group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week Antisterone equivalent to 20?mg·kg 1 ·d 1 was given intragastrically (ig) In the malotilate group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week Malotilate equivalent to 50?mg·kg 1 ·d 1 was given ig In the control group (n=40), the rats were injected with olive oil only After 2,4,6,8 and 10 weeks, animals were sacrificed, and morphological examination was carried out The area of collagen was examined with an Image Analyse System Expression of the aldosterone synthase gene, CYP11B2 mRNA, in fibrotic and normal liver was detected by means of reverse transcriptase polymerase chain reaction (RT PCR) and in situ hybridization Results In situ hybridization and RT PCR showed that the expression of CYP11B2 mRNA, which localized in the endoplasm of hepatic stellate cells (HSCs), was up regulated when fibrogenesis occurred Histological observation indicated that the grade of fibrosis and the area of collagen in the antisterone group were less than those in model group before 6 weeks ( P <0 05) There was no significant difference between the antisterone and malotilate groups ( P >0 05) After that, however, the grade of fibrosis and the area of collagen in the antisterone group were higher than those in the malotilate group ( P <0 05) There was no significant difference between the antisterone and model groups ( P >0 05) Conclusions The expression of CYP11B2 mRNA is up regulated in fibrotic liver Antisterone can have a partial fibrogenesis identify aldosterone synthase gene CYP11B2 mRNA expression in normal and fibrotic liver in rats and evaluate the curative effect of antisterone Methods 160 Wistar rats weighing about 250?g were divided into 4 groups In the model group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week In the antisterone group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week Antisterone equivalent to 20?mg·kg 1 ·d 1 was given intragastrically (ig) In the malotilate group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week Malotilate equivalent to 50?mg·kg 1 ·d 1 was given ig In the control group (n=40), the rats were injected with olive oil only After 2,4,6,8 and 10 weeks, animals were sacrificed, and morphological examination was carried out The area of collagen was examined with an Image Analyse System Expression of the aldosterone synthase gene, CYP11B2 mRNA, in fibrotic and normal liver was detected by means of reverse transcriptase polymerase chain reaction (RT PCR) and in situ hybridization Results In situ hybridization and RT PCR showed that the expression of CYP11B2 mRNA, which localized in the endoplasm of hepatic stellate cells (HSCs), was up regulated when fibrogenesis occurred Histological observation indicated that the grade of fibrosis and the area of collagen in the antisterone group were less than those in model group before 6 weeks ( P <0 05) There was no significant difference between the antisterone and malotilate groups ( P >0 05) After that, however, the grade of fibrosis and the area of collagen in the antisterone group were higher than those in the malotilate group ( P <0 05) There was no significant difference between the antisterone and model groups ( P >0 05) Conclusions The expression of CYP11B2 mRNA is up regulated in fibrotic liver Antisterone can have a partial fibrogenesis

关 键 词：CYP11B2 gene hepatic fibrogenesis hepatic stellate cells antisterone in situ hybridization reverse transcriptase polymerase chain reaction 

分 类 号：R394[医药卫生—医学遗传学]