Telomerase activity and homozygous deletions of the p16 gene in liver metastases of colorectal carcinoma  

大肠癌肝转移肿瘤中端粒酶活性和p16基因纯合缺失(英文)

作　　者：马晋平[1] 詹文华[1] 彭俊生[1] 何裕隆[1] 郑章清[1] 蔡世荣[1] 王磊[1] 汪建平[1] 

机构地区：[1]中山医科大学附属第一医院胃肠胰外科,广州510080

出　　处：《Chinese Medical Journal》2001年第10期60-64,108,共6页中华医学杂志（英文版）

基　　金：ThisstudywassupportedbytheDoctorStartupFoundationofGuangdongProvince (No 9940 0 7);theMedicalScienceFoundationofGuangdongProvince (No A199914 7)

摘　　要：Objective To study the interaction between telomerase activity and abnormalities of the p16 gene in liver metastases of colorectal carcinoma.Methods Telomerase activity was detected by a non-isotopic PCR-based telomeric repeat amplification protocol (TRAP) assay, and homozygous deletions of the p16 gene were detected by a semiquantitative multiplex polymerase chain reaction in tissue samples from 24 liver metastases of colorectal carcinoma and 5 primary colorectal carcinomas.Results Telomerase activity was observed in 19 (79.2%) of 24 liver metastases of colorectal carcinoma.Telomerase activity was also observed in all 5 primary colorectal carcinomas and in 3 of their liver metastatic samples. The incidence of telomerase activity in liver metastases of colorectal carcinoma was not significantly correlated to tumor diameter, number of tumors, cirrhosis, and HBsAg. Homozygous deletions of the p16 gene were found in 9 of 24 (37.5%) liver metastases of colorectal carcinoma. Homozygous deletions of the p16 gene were observed in 2 of the 5 primary colorectal carcinomas and in 1 of the matching liver metastatic cancers. There was a correlation between telomerase activity and homozygous deletions of the p16 gene.Conclusions There is a correlation between telomerase activity and homozygous deletions of the p16gene in liver metastases of colorectal carcinoma, suggesting its crucial role in liver metastases. However,telomerase activation and homozygous deletions of the p16 gene might not be the initiating event in liver metastases of colorectal carcinoma.目的　研究人大肠癌肝转移肿瘤中端粒酶活性和p16基因异常及其相互关系 ,并探讨其临床意义。方法　采用TRAP银染方法和半定量多重PCR分别检测了 2 4例大肠癌肝转移肿瘤组织包括其中 5例原发大肠癌组织端粒酶活性及p16基因的纯合缺失情况 ,并结合临床病理参数 ,进行统计学分析。结果　本组 2 4例大肠癌肿转移肿瘤组织中检测到 19例端粒酶阳性 ,阳性率为 79 2 %。 5例原发大肠癌均检测到端粒酶活性 ,其中 3例在相应的肝转移组织中也检测到端粒酶活性。端粒酶活性与转移瘤大小、肝内转移灶数目、HBsAg是否伴有肝纤维化无关。在 2 4例转移瘤组织中有 9例标本中检测到p16基因纯合缺失 ,缺失率为37 5 %。 5例原发大肠癌有 2例检测p16基因的纯合性缺失 ,其中 1例患者在原发肿瘤和转移瘤均检测到p16基因的纯合性缺失。p16基因的纯合缺失与端粒酶活性相关。结论　大肠癌肝转移肿瘤中端粒酶活性和p16基因异常对阐明大肠癌转移的生物学行为可能有重要的意义。端粒酶的异常激活和p16基因异常在大肠癌肝转移过程中可能不是早期事件。

关 键 词：telomerase activity  ·    p16 gene  ·   liver metastases  ·   colorectal carcinoma 

分 类 号：R735.34[医药卫生—肿瘤]