Histopathological changes of Macaca mulatta infected with Plasmodium knowlesi  

恒河猴感染诺氏疟原虫的病理组织学变化(英文)

作　　者：陈林[1] 李国桥[1] 卢耀增[1] 罗灼玲[2] 

机构地区：[1]广州中医药大学热带医学研究所,广州510405 [2]广州中医药大学组织胚胎学教研室,广州510405

出　　处：《Chinese Medical Journal》2001年第10期65-69,108,共6页中华医学杂志（英文版）

摘　　要：Objective To study the histopathological changes of relevant intemal organs of Macaca mulatta infected with Plasmodium knowlesi ( P. knowlesi).Methods Histopathological examination of 3 monkeys who died of P. knowlesi infection, 2 P. knowlesi infected monkeys who died of treatment failure with artesunate suppository and 1 P. knowlesi infected monkey that was cured by piperaquine phosphate (PQP) but died of trauma and necrosis of the fore limb.Results The heart, liver, spleen, lung, kidney, brain, pancreas, parathyriod, pituitary and lymph nodes showed severe pathological changes in 3 monkeys (No. 1, 7 and 12) who died of P. knowlesi infection and 1 infected monkey (No. 72) who died of treatment failure with artesunate suppository. Red blood cells containing rmalarial parasites and pigments were concentrated in the capillaries of these organs. Malarial pigments were deposited in many organs or phagocytized by macrophages in 1 monkey (No. 131 ), it was cured by piperaquine phosphate but died of trauma and necrosis of the fore limb; cellular atrophy and disappearance of pancreatic islets, parathyroid and pituitary cells were also observed. One monkey (No.33) treated with artesunate suppository, showed that blood parasites became negative but recrudesced and pituiary later died from a gavage accident. Its organs showed a significant difference to those of the infected monkeys receiving no treatment. Only the liver Kupffer cells and cerebral matrix contained malarial parasites and pigments; many relevant intemal organs showed repair.Conclusion The pathological changes of relevant internal organs of Macaca mulatta infected with P. knowlesi were examined in detail, especially cellular atrophy and the disappearance of pancreatic islets, parathyoid and pituitary cells and myolysis of cardiac muscles. These changes have not previously been reported elsewhere.目的　探讨诺氏疟原虫 (Plasmodiumknowlesi)感染恒河猴 (Macacamulatta)引起有关脏器的病理组织学变化。方法　病解 3例感染疟原虫未经治疗病死的猴 ;2例感染疟原虫经青蒿琥酯 (artesunate)栓剂治疗后又复发的猴 ;1例感染疟原虫经磷酸哌喹 (piperaquinephosphate)治愈后死于上肢坏死感染的猴。对有关脏器均进行切片检查。结果　 3例 (1号、7号和 12号 )未经治疗而病死的猴和 1例 (72号 )虽经青蒿琥酯栓剂治疗但很快被排出体外的猴的心、肝、脾、肺、肾、大小脑、胰腺、甲状旁腺、脑垂体和淋巴结等均有严重病变 ,含原虫的红细胞和疟色素聚集于这些脏器的毛细血管内。 1例 (131号 )经用哌喹治愈而死于上肢坏死的猴 ,除见多个脏器内有色素沉积或被巨噬细胞吞噬外 ,可见胰岛细胞、甲状旁腺和垂体细胞萎缩和消失等病变。 1例 (33号 )用青蒿琥酯栓剂治疗后短期原虫转阴 ,但又复燃的猴被灌药致死 ,多个脏器所见与感染后未经治疗者明显不同 ,仅见肝脏Kupffer细胞和大脑基质细胞内含原虫和疟色素 ,多个脏器均呈修复。结论　本文报导了诺氏疟原虫感染恒河猴后各有关脏器的病理组织学变化 ,特别是观察到胰岛细胞、甲状旁腺和垂体细胞的萎缩和消失与心肌细胞的溶解是国内外文献所未记载过的。

关 键 词：Plasmodium knowlesi   ·  Macaca  mulatta   ·    necropsy  ·   histopathology 

分 类 号：R361[医药卫生—病理学]