Mouse neural stem cells cultured in vitro and expressing an exogenous gene  

作　　者：Jiong Tang Hong Yu Lizhu Lin Haoquan Wu Shouyuan Zhao Changben Li 

机构地区：[1]Fudan Univ, Inst Genet, Sch Life Sci, Shanghai 200433, Peoples R China

出　　处：《Chinese Science Bulletin》2001年第7期566-567,617,共3页

基　　金：the fund of ClimbingProgram provided by the State Science and Technology Commission of China.

摘　　要：Neural stem cells are the multipotential, self-renewing cells in central nerve system, and play an essential role in the development and differentiation of nerve system. Neural stem cells can be used to treat the nerve system diseases, especially, the transplantation of neural stem cells to rescue the degenerated neural cells has become a very promising therapeutic way. We successfully cultured neural stem cells isolated from the brains of embryonic mice in vitro and determined their distribution in the E17 mice brains. The neural stem cells were transfected with adenoviral vector carrying GFP (green fluorescence protein) gene and then highly expressed the exogenous gene. It paves the way for gene therapy of degenerative nerve system diseases.Neural stem cells are the multipotential, self-renewing cells in central nerve system, and play an essential role in the development and differentiation of nerve system. Neural stem cells can be used to treat the nerve system diseases, especially, the transplantation of neural stem cells to rescue the degenerated neural cells has become a very promising therapeutic way. We successfully cultured neural stem cells isolated from the brains of embryonic micein vitro and determined their distribution in the E17 mice brains. The neural stem cells were transfected with adenoviral vector carrying GFP (green fluorescence protein) gene and then highly expressed the exogenous gene. It paves the way for gene therapy of degenerative nerve system diseases.

关 键 词：neural stem cells IMMUNOHISTOCHEMICAL ASSAY adenovi-ral vector. 

分 类 号：Q42[生物学—神经生物学]