Expression detection and partial cloning of porcine leptin receptor (OBR) gene  

作　　者：HU Xiaoxiang DAI Rujuan LI Ming WU Changxin 

机构地区：[1]College of Animal Science and Technology,China Agricultural University,Beijing 100094,China [2]National Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100094,China

出　　处：《Chinese Science Bulletin》2001年第5期396-400,共5页

基　　金：the National Natural Science Foundation of China (Grant No. 39725022 ) ; the State Key Basic Research Development Program (Grant No. 2000016100).

摘　　要：The product of the obesity gene, called leptin, is an important regulator of adiposity, which mainly functions via its regulation of feed intake and energy metabolism. Both obesity gene (ob gene) and leptin receptor gene (OBR gene) are thought to play a major role in controlling of body fat mass as well as body weight. The result of RT-PCR shows that levels of pig OBR mRNA are higher in hypothalamus, lung and liver, and lower expression can be detected in other tissues. Total RNA purified from 11 different organs and tissues have been hybridized with pig OBR cDNA probes. The hybridization signals are shown in 7 organs and tissues. 4.1 and 3.8 kb bands were observed from hypothalamus, whereas 3.8 and 3.5 kb bands were observed in other tissues instead. The nearly complete sequence of the extracellular domain of pig OBR gene was obtained. The homology of sequence is 89.2% between pig and human, 80.3% between pig and mouse. Alignment of the predicted ammo acid sequence of OBR in pig, human and mouse showsThe product of the obesity gene, called leptin, is an important regulator of adiposity, which mainly functions via its regulation of feed intake and energy metabolism. Both obesity gene (ob gene) and leptin receptor gene (OBR gene) are thought to play a major role in controlling of body fat mass as well as body weight. The result of RT-PCR shows that levels of pigOBR mRNA are higher in hypothalamus, lung and liver, and lower expression can be detected in other tissues. Total RNA purified from 11 different organs and tissues have been hybridized with pigOBR cDNA probes. The hybridization signals are shown in 7 organs and tissues. 4.1 and 3.8 kb bands were observed from hypothalamus, whereas 3.8 and 3.5 kb bands were observed in other tissues instead. The nearly complete sequence of the extracellular domain of pigOBR gene was obtained. The homology of sequence is 89.2% between pig and human, 80.3% between pig and mouse. Alignment of the predicted amino acid sequence ofOBR in pig, human and mouse shows that the overall identity is 86.5% between pig and human, 76.6% between pig and mouse. Two WSXWS motifs were found at aa313and aa6162.

关 键 词：PIG ob (obese) GENE OBR (leptin receptor) GENE LEPTIN RT-PCR Northern blotting. 

分 类 号：S828[农业科学—畜牧学]