Immunohistochemical localization of Ca^(2+)/calmodulin-dependent kinase in tobacco  

作　　者：YANG Wannian, LIANG Shuping & LU YingtangCenter of Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China 

出　　处：《Chinese Science Bulletin》2001年第3期230-233,共4页

基　　金：This work was supported in part by the National Science Fund for Outstanding Youth (Grant No. 39725015); the National Natural Science Foundation of China (Grant No. 39780015); the National Basic Research Program (Grant No. G19990116) ;and the National

摘　　要：The existence of Ca2+/calmodulin-dependent kinase (CaM kinase, CaMK) in tobacco is verified immuno-logically and its distribution in different tissues of tobacco is studied. It has been demonstrated that CaMK is mainly distributed in early developing anthers, developing ovules and embryos, lateral root primordium, apical meristem and leaf primordium of buds and mesophyll cells and developing vascular bundles of leaves. There is enormous CaM kinase distributed in leaf epidermis fair cells and guard cells of stomas too. Little kinase is found in mature stem or root cells. The distribution properties of CaM kinase in tobacco are consistent with those of CaM, suggesting that there exists the Ca2+ signal transduction pathway mediated by CaM kinase in tobacco and it plays an important role in the plant growth and development.The existence of Ca2+/calmodulin-dependent kinase (CaM kinase, CaMK) in tobacco is verified immuno-logically and its distribution in different tissues of tobacco is studied. It has been demonstrated that CaMK is mainly distributed in early developing anthers, developing ovules and embryos, lateral root primordium, apical meristem and leaf primordium of buds and mesophyll cells and developing vascular bundles of leaves. There is enormous CaM kinase distributed in leaf epidermis fair cells and guard cells of stomas too. Little kinase is found in mature stem or root cells. The distribution properties of CaM kinase in tobacco are consistent with those of CaM, suggesting that there exists the Ca2+ signal transduction pathway mediated by CaM kinase in tobacco and it plays an important role in the plant growth and development.

关 键 词：CaM KINASE IMMUNOHISTOCHEMICAL localization TOBACCO anthers. 

分 类 号：TS41[农业科学—烟草工业]