Cloning and analysis of a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea  

作　　者：XU Huasong XU Yunjian GU Xuesong HU Tingzhang SU Yanhui ZHU Yuxian 

机构地区：[1]The National Laboratory of Protein Engineering and Plant Genetic Engineering,College of Life Sciences,Peking University,Beijing 100871,China [2]College of Biotechnology,South China Agricultural University,Guangzhou 510642,China [3]Department of Biology,Sichuan Three-Gorge College,Chongqing 404000,China

出　　处：《Chinese Science Bulletin》2001年第21期1808-1812,共5页

基　　金：This work was supported by the National Science Foundation for Distinguished Young Scholars (Grant No. 39725002); and the National "863" High-Tech Project (Grant No. 101-01-01-02).

摘　　要：Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GAa application. Screening a G2 pea cDNA library using this fragment as a probe, we obtained a 2036 bp full-length cDNA. It contains a 1746 bp open reading frame and encodes a protein of 581 amino acids with a theoretical molecular weight of 64 ku. It shares high-level sequenceidentity with AAIR genes from other plant species. This cDNA was cloned into expression vector and recombinant E. coli DH5α cells with remarkable AAIR enzyme activity were obtained.Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GA3 application. Screening a G2 pea cDNA library using this fragment as a probe, we obtained a 2036 bp full-length cDNA. It contains a 1746 bp open reading frame and encodes a protein of 581 amino acids with a theoretical molecular weight of 64 ku. It shares high-level sequence identity withAAIR genes from other plant species. This cDNA was cloned into expression vector and recombinantE. coli DH5α cells with remarkable AAIR enzyme activity were obtained.

关 键 词：G2 PEA CLONE acetohydroxy ACID isomeroreductase PROKARYOTIC expression. 

分 类 号：Q785[生物学—分子生物学]