Expression analysis of gdcsP promoter from C_3-C_4 intermediate plant Flaveria anomala in transgenic rice  

作　　者：CHEN Shuai, QU Nan, CAO Shouyun, Hermann Bauwe. CHEN Shouyi, TIAN Wenzhong & CHU Chengcai1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 2. Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany 3. Rostock University, 18055 Rostock, Germany 

出　　处：《Chinese Science Bulletin》2001年第19期1635-1638,1675,共5页

基　　金：This work was supported by K. -C. Wang Foundation and Hundred-of-Talent Project of CAS.

摘　　要：The gdcsP promoter isolated from C3-C4 intermediate plant Flaveria anomala was fused to the β-glu-curonidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced into the rice (Oryza saliva L.) cv. 8706 by Agrobacterium-mediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However, no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression declined significantly with the maturity of plants. These expression patterns make the gdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues.The gdcsP promoter isolated from C3-C4 intermediate plant Flaveria anomala was fused to the β-glu-curonidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced into the rice (Oryza saliva L.) cv. 8706 by Agrobacterium-mediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However, no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression declined significantly with the maturity of plants. These expression patterns make the gdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues.

关 键 词：rice (Oryza sativa L.) glycine decarboxyla.se P-PROTEIN SUBUNIT vascular tissue. 

分 类 号：S511[农业科学—作物学]