Identification of selenocyst-eine insertion sequence(SECIS) element in eukaryotic selenoproteins by RNA Draw program  

作　　者：XU Huibi HUANG Kaixun QU Xianghu GAO Zhonghong LIU Qiong CHEN Runsheng XUAN Zhenyu 

机构地区：[1]Department of Chemistry,Huazhong University of Science and Technology,Wuhan 430074,China [2]Department of Protein Engineering,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Chinese Science Bulletin》2001年第14期1159-1161,共3页

摘　　要：The computer program RNA Draw was used to identify the secondary structures in the 3’untranslated regions (3’UTRs) of the mRNAs from 46 eukaryotic seleno-proteins among 7 species. The program found one or two possible SECIS elements in these selenoproteins. The SECIS element consists of a stem-loop or hairpin structure with three conserved sequences of AUGA-(A)AA-GA. SECIS element was not found by the RNA Draw program in randomly selected non-selenoproteins. The results showed that SECIS element is the unique character of the genes ofeukaryotic selenoproteins. Thus it is possible to use RNA Draw to search the SECIS elements in gene bank for potential new selenoproteins.The computer program RNA Draw was used to identify the secondary structures in the 3′ untranslated regions (3′UTRs) of the mRNAs from 46 eukaryotic selenoproteins among 7 species. The program found one or two possible SECIS elements in these selenoproteins. The SECIS element consists of a stem-loop or hairpin structure with three conserved sequences of AUGA-(A)AA-GA. SECIS element was not found by the RNA Draw program in randomly selected non-selenoproteins. The results showed that SECIS element is the unique character of the genes of eukaryotic selenoproteins. Thus it is possible to use RNA Draw to search the SECIS elements in gene bank for potential new selenoproteins.

关 键 词：selenoprotein eukaryotes 3’ untranslated region (3’ UTR) SELENOCYSTEINE insertion sequence (SECIS) RNA DRAW program. 

分 类 号：Q522[生物学—生物化学]