Three-dimensional structure of the wild-type RHDV  

作　　者：ZHENG Dong XUE Tao CHEN Donghua ZHENG Ming Z.H.ZHOU XU Wei 

机构地区：[1]Beijing Laboratory of Electron Microscopy,Institute of Physics,Chinese Academy of Sciences,Beijing 100080,China [2]Department of Pathology and Laboratory Medicine,University of Texas-Houston Medical School,Houston,TX 77030,USA [3]Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [4]Analytical and Testing Center,Beijing Normal University,Beijing 100875,China [5]China Institute of Veterinary Drug Control,Beijing 100081,China

出　　处：《Chinese Science Bulletin》2001年第12期1005-1009,共5页

基　　金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 69501008 and 19634020); Research in Houston has been supported by NIH (AI 46420) and the Welch Foundation (AU-1492).

摘　　要：The three-dimensional (3D) structure of the wild-type rabbit hemorrhagic disease virus (RHDV) has been determined to a resolution of 3.2 nm by electron cryo-microscopy and computer image reconstruction techniques. The 3D density map exhibits characteristic structural features of a calicivirus: a T=3 icosahedral capsid with 90 arch-like capsomeres at the icosahedral and local 2-fold axes and 32 large surface hollows at the icosahedral 5- and 3-fold axes. This result confirms that the RHDV isolated in China is a member of the Caliciviridae family. A rather continuous capsid shell was found without channels. However, our RHDV structure also reveals some distinct structural char-acteristics not observed in other caliciviruses, including interconnected capsomeres and the lack of protuberance on the base of each of the surface hollows. Two types of particles were identified with similar outer capsid structure but different density distributions inside the capsid shells, which could not be distinguished byThe three-dimensional (3D) structure of the wild-type rabbit hemorrhagic disease virus (RHDV) has been determined to a resolution of 3.2 nm by electron cryomicroscopy and computer image reconstruction techniques. The 3D density map exhibits characteristic structural features of a calicivirus: a T=3 icosahedral capsid with 90 arch-like capsomeres at the icosahedral and local 2-fold axes and 32 large surface hollows at the icosahedral 5- and 3-fold axes. This result confirms that the RHDV isolated in China is a member of the Caliciviridae family. A rather continuous capsid shell was found without channels. However, our RHDV structure also reveals some distinct structural characteristics not observed in other caliciviruses, including interconnected capsomeres and the lack of protuberance on the base of each of the surface hollows. Two types of particles were identified with similar outer capsid structure but different density distributions inside the capsid shells, which could not be distinguished by conventional negative staining electron microscopy. As the genomic and subgenomic RNAs are both packaged into particles for RHDV, we suggest that the two types of particles identified correspond to those containing either the genomic or subgenomic RNAs, respectively.

关 键 词：ELECTRON CRYOMICROSCOPY THREE-DIMENSIONAL reconstruction RHDV calicivirus. 

分 类 号：R-39[医药卫生]