Ultrastructural localization of active genes in Allium cepa cells  

作　　者：TAO Wei HE Mengyuan HAO Shui 

机构地区：[1]Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China [2]Department of Cell Biology,College of Life Sciences,Peking University 100871,China

出　　处：《Chinese Science Bulletin》2001年第11期925-930,共6页

基　　金：the National Natural Science Foundation of China (Grant No. 39770367) and the Special Funds for the National Major Basic Research Program of China (Grant No. G19990539).

摘　　要：By using the anti-RNA/DNA hybrid antibody as the probe, we labeled and analyzed the precise transcrip-tional sites of active genes in Allium cepa cells in situ. The results showed that the location of labeled signals appeared in mitochondrion was the same as that in chloroplast, generally concentrated at the central matrix space where there were no cristae and thylakoids. In the extranucleolar regions of nucleus, the labeled signals of transcriptional sites were situated at the perichromatin fibrils, which decondensed and stretched out from the chromosome territories. Our results also displayed the concentrations of labeled signals in a certain region of nucleus, and this means that the gene transcription rich region actually existed in Allium cepa cells. In nucleolus, the synthetic sites of rRNA were localized not only to the periphery of fibrillar centers but also to the DFC near FC.By using the anti-RNA/DNA hybrid antibody as the probe, we labeled and analyzed the precise transcriptional sites of active genes inAllium cepa cellsin situ. The results showed that the location of labeled signals appeared in mitochondrion was the same as that in chloroplast, generally concentrated at the central matrix space where there were no cristae and thylakoids. In the extranucleolar regions of nucleus, the labeled signals of transcriptional sites were situated at the perichromatin fibrils, which decondensed and stretched out from the chromosome territories. Our results also displayed the concentrations of labeled signals in a certain region of nucleus, and this means that the gene transcription rich region actually existed inAllium cepa cells. In nucleolus, the synthetic sites of rRNA were localized not only to the periphery of fibrillar centers but also to the DFC near FC.

关 键 词：ALLIUM CEPA CHROMOSOME TERRITORIES NUCLEOLUS extranu- cleolar region of nuclei active gene transcription. 

分 类 号：R392.1[医药卫生—免疫学]