PcTGD,a highly expressed gene in stem,is related to water stress in reed (Phragmites communis Trin.)  

作　　者：CHENG Youfa PU Tongliang XUE Yongbiao ZHANG Chenglie 

机构地区：[1]Institute of Botany&Plant Physiology,School of Life Science of Lanzhou University,Lanzhou 730000,China [2]Institute of Developmental Biology,Chinese Academy of Sciences,Beijing 100080,China

出　　处：《Chinese Science Bulletin》2001年第10期850-854,共5页

基　　金：This work was supported by the State Basic Research Program (Grant No. G1999011705) ;and the National NaturalScience Foundation of China (Grant No. 39870062).

摘　　要：To compare differential gene expression among three ecotypes of reed (Phragmites communis Trin.), dune reed (DR), heavy-salt meadow reed (HSR) and light-salt meadow reed (LSR), mRNA transcripts were displayed by cDNA-AFLP (amplified fragment length polymorphisms). The result revealed that a relatively small number of genes are likely involved in adaptations of DR and HSR to stresses. A full-length cDNA encoding dTDP-D-glucose dehydratase gene (PcTGD) was subsequently cloned from DR. Northern blot analysis showed that it is highly expressed in stem as well as rhizoma of the three ecotypes. However, its expression in DR stem was much higher than that of the other two ecotypes. After the removal of water stress, the expression of PcTGD was significantly reduced, suggesting that it possibly plays a role in adaptation of DR to water stress through an osmotic regulation mechanism.To compare differential gene expression among three ecotypes of reed (Phragmites communis Trin.), dune reed (DR), heavy-salt meadow reed (HSR) and light-salt meadow reed (LSR), mRNA transcripts were displayed by cDNA-AFLP (amplified fragment length polymorphisms). The result revealed that a relatively small number of genes are likely involved in adaptations of DR and HSR to stresses. A full-length cDNA encoding dTDP-D-glucose dehydratase gene (PcTGD) was subsequently cloned from DR. Northern blot analysis showed that it is highly expressed in stem as well as rhizoma of the three ecotypes. However, its expression in DR stem was much higher than that of the other two ecotypes. After the removal of water stress, the expression ofPcTGD was significantly reduced, suggesting that it possibly plays a role in adaptation of DR to water stress through an osmotic regulation mechanism.

关 键 词：REED (Phragmites communis Trin.) water stress dTDP-D-glucose DEHYDRATASE (PcTGD). 

分 类 号：Q947[生物学—植物学]