Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization  

作　　者：Li Ming DU Zhe Feng FAN Jin Li QIAO Jing Ping WANG 

机构地区：[1]Analysis and Test Center,Shanxi Teachers University,Lingfen 041004 [2]Department of Chemistry,Arts and Sciences College,Taiyuan University of Technology,Taiyuan 030006

出　　处：《Chinese Chemical Letters》2001年第11期1007-1010,共4页中国化学快报（英文版）

摘　　要：Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%similar to 95.7% and the RSD is 1.2%similar to4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%similar to 95.7% and the RSD is 1.2%similar to4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.

关 键 词：SPARFLOXACIN HPLC pre-column derivatization human urine 

分 类 号：O657.7[理学—分析化学]