Preliminary crystallographic studies on two insulin analogues which retain high biological activities after alterations in A chain  

作　　者：Yiding Huang Hui Chen Youmin Feng Jiping Zhang 

机构地区：[1]Shanghai Inst Biochem, State Key Lab Mol Biol, Shanghai 200031, Peoples R China [2]Inst Biophys, State Key Lab Biomacromol, Beijing 100101, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第16期1480-1483,共4页

摘　　要：Using Fmoc solid phase synthesis and site-directed gene mutagenesis, two insulin analogues, [A13-14GABA,A21Ala] porcine insulin and [A3Thr] human insulin, have been prepared respectively, which retain high biological activities. The results show that non-coded y-amino butyric acid (GABA) could replace the dipeptide, Leu-Tyr, in A13-A14 of insulin andUsing Fmoc solid phase synthesis and site-directed gene mutagenesis, two insulin analogues, [A13-14GABA, A21Ala] porcine insulin and [A3Thr] human insulin, have been prepared respectively, which retain high biological activities. The results show that non-coded yamino butyric acid (GABA) could replace the dipeptide, Leu-Tyr, in A13-A14 of insulin and that the hydrophilic Thr could substitute Val in A3 of insulin which is highly conservative and included in the receptor binding site. The crystal culture and preliminary crystallographic studies by X-ray diffraction on these two insulin analogues are reported.

关 键 词：[A13-14GABA.A21Ala] PORCINE INSULIN [A3Thr] human INSULIN INSULIN ANALOGUE crystal culture X-ray diffraction. 

分 类 号：Q57[生物学—生物化学]