Apoptosis of carrot nuclei in in vitro system induced by cytochrome c  

作　　者：Yun Zhao Yingli Sun Zhengfan Jiang Zhonghe Zhai 

机构地区：[1]Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第16期1497-1502,共6页

摘　　要：The apoptosis of carrot nuclei induced in cytosol of carrot cells by cytochrome c is reported. This kind of cell-free apoptosis system of plant, CS-100, is an efficient In vitro inducing system. Several typical characteristics of apoptosis including chromatin condensation, margina-tion and apoptotic bodies could be detected, and the degradation of genome in carrot nucleiThe apoptosis of carrot nuclei induced in cytosol of carrot cells by cytochrome c is reported. This kind of cell-free apoptosis system of plant, CS-100, is an efficientin vitro inducing system. Several typical characteristics of apoptosis including chromatin condensation, margination and apoptotic bodies could be detected, and the degradation of genome in carrot nuclei into nucleosomal fragments could be detected. TUNEL assay shows the specific breakage of 3′-OH ends of DNA strand. The results indicate that CS-100 is an efficient and easily-operatedin vitro system to study the apoptotic mechanism in plant and the apoptosis in plant cells may share similar pathways to apoptosis in animal cells.

关 键 词：APOPTOSIS CYTOCHROME c CYTOSOL CELL-FREE system CARROT nuclei. 

分 类 号：S631.2[农业科学—蔬菜学]