Effect of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia  

作　　者：Dongcheng Lu Zhengmao Ye Xuejun Tian Guanghui Chen Jian Tang Guoying Zhu Airu Zhou 

机构地区：[1]Beijing Med Univ, Inst Cardiovasc Res, Beijing 100083, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第15期1391-1397,共7页

摘　　要：To investigate the feasibility of intramuscular electro-acupuncture-mediated VEGF gene transfer on experimental limb ischemia, the transcription and expression of VEGF gene have been detected with RT-PCR and immunohistochemistry staining, and the biological effect of VEGF gene inside the nat limb has been observed through vascular angiography. Results from angiography show that the transfer of VEGF gene into the rat with experimental limb ischemia can stimulate the formation of neovessel, establish collateral circulation, augment blood perfusion, and ameliorate distal limb necrosis. These findings reveal that intramuscular electro acupuncture-mediated VEGF gene transfer may present a new therapeutic modality for treating tissue ischemia.To investigate the feasibility of intramuscular electro-acupuncture-mediatedVEGF gene transfer on experimental limb ischemia, the transcription and expression ofVEGF gene have been detected with RT-PCR and immunohistochemistry staining, and the biological effect ofVEGF gene inside the nat limb has been observed through vascular angiography. Results from angiography show that the transfer ofVEGF gene into the rat with experimental limb ischemia can stimulate the formation of neovessel, establish collateral circulation, augment blood perfusion, and ameliorate distal limb necrosis. These findings reveal that intramuscular electroacupuncture-mediatedVEGF gene transfer may present a new therapeutic modality for treating tissue ischemia.

关 键 词：ELECTRO-ACUPUNCTURE gene TRANSFER VASCULAR endothellal growth FACTOR occluslve angitis. 

分 类 号：R245[医药卫生—针灸推拿学]