Cloning and characterization of a C genome-specific repetitive sequence in Aegilops caudata L.  

作　　者：Xiuying Kong Ronghua Zhou Yuchen Dong Jizeng Jia 

机构地区：[1]Chinese Acad Agr Sci, Minist Agr, Key Lab Crop Germplasm & Biotechnol, Beijing 100081, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第14期1301-1306,共6页

摘　　要：pAeca212 is 204 bp in length, and the G + C content is 51%. It disperses on all seven chromosome pairs of Aegilops caudata except centromeres and secondary constrictions.Compared with the 316893 DNA sequences registered in Genbank/EMBL/DDJB/PDB, pAeca212 is a new C-genome specific repetitive sequence. The results of genomic specificity analysis of pAeca212 show that there are no hybridization signals detected in all donor Poaceae plants except in rye. pAeca212 is a very useful molecular marker in the study of the origin of Triticeace and the detection of C chromatin in wheat background.pAeca212 is 204 bp in length, and the G + C content is 51%. It disperses on all seven chromosome pairs ofAegilops caudata except centromeres and secondary constrictions. Compared with the 316893 DNA sequences registered in Genbank/EMBL/DDJB/PDB, pAeca212 is a new C-genome specific repetitive sequence. The results of genomic specificity analysis of pAece212 show that there are no hybridization signals detected in all donor Poaceae plants except in rye. pAeca212 is a very useful molecular marker in the study of the origin of Triticeace and the detection of C chromatin in wheat background.

关 键 词：AEGILOPS CAUDATA GENOME SPECIFIC REPETITIVE sequence. 

分 类 号：Q943[生物学—植物学]