Synthesis and energy transfer of model conjugate of phycobilisome rods  

作　　者：Jiquan Zhao Jingquan Zhao Lijin Jiang Jingmin Zhang Fuli Zhao Xiguang Zheng Hezhou Wang 

机构地区：[1]Chinese Acad Sci, Inst Photog Chem, Beijing 100101, Peoples R China [2]Zhongshan Univ, Inst Laser & Spectra, Guangzhou 510275, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第2期134-139,共6页

摘　　要：The conjugate of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) was synthesized through a heterobifunctional coupling reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. The molar ratio of R-PE to C-PC was determined by absorption spectra, and the result was 2:1. The energy transfer phenomena were observed from steady-state fluorescence spectra. The calculated result showed that the energy transfer efficiency from R-PE to C-PC was 88%. The energy transfer kinetics was determined by picosecond time-resolved fluorescence spectra. The time constant of energy transfer from R-PE to C-PC was 80 ps, which was much longer than that in the rood of native phycobilisomes.The conjugate of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) was synthesized through a heterobifunctional coupling reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. The molar ratio of R-PE to C-PC was determined by absorption spectra, and the result was 2:1. The energy transfer phenomena were observed from steady-state fluorescence spectra. The calculated result showed that the energy transfer efficiency from R-PE to C-PC was 88%. The energy transfer kinetics was determined by picosecond time-resolved fluorescence spectra. The time constant of energy transfer from R-PE to C-PC was 80 ps, which was much longer than that in the rod of native phycobilisomes.

关 键 词：R-PHYCOERYTHRIN C-PHYCOCYANIN rod MODEL of PHYCOBILISOMES energy transfer time-resolved fluorescence spectra 

分 类 号：Q6[生物学—生物物理学]