R-PHYCOERYTHRIN

作品数:15被引量:8H指数:2
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相关领域:生物学理学更多>>
相关作者:朱丽萍张玉忠颜世敢王广策孙海宝更多>>
相关机构:山东轻工业学院山东大学山东省农业科学院邹平县畜牧兽医局更多>>
相关期刊:《Science China(Life Sciences)》《Science China Chemistry》《西南农业学报》《Science Bulletin》更多>>
相关基金:国家自然科学基金中国科学院知识创新工程王宽诚教育基金国家重点实验室开放基金更多>>
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R-phycoerythrin标记抗猪瘟病毒荧光抗体的制备及其与FITC标记荧光抗体检测的比较研究被引量:2
《西南农业学报》2011年第5期1962-1966,共5页赵守山 颜世敢 朱丽萍 崔道实 
国家高技术研究发展计划(2002AA302213);兽医生物技术国家重点实验室开放基金(SKLVBF200909)
用摩尔比100∶1的化学交联剂SPDP分别将RPE、抗猪瘟病毒抗体衍生,两种衍生物再以摩尔比1∶1液相交联,HPLC纯化制备出RPE标记的抗猪瘟病毒荧光抗体。对制备的荧光抗体从荧光特性、抗体活性、稳定性及纯度等方面进行鉴定。以溴化氰活化的...
关键词:R-藻红蛋白 荧光标记 固相免疫荧光检测 猪瘟病毒 
Cloning and Sequencing the y Subunit of R-Phycoerythrin from Corallina officinalis
《Acta Botanica Sinica》2004年第10期1135-1140,共6页WANGSheng ZHONGFu-Di WUZu-Jian LINQi-Ying XIELian-Hui 
The full-length cDNA of the γ subunit of R-phycoerythrin from Corallina officinalis L. was cloned by rapid amplification of cDNA ends (RACE) method, and sequenced. The full-length cDNA is a 2 308 bp consisting of 5' ...
关键词:R-藻红蛋白 红藻 蓝藻细菌 克隆 序列分析 珊瑚藻属 
Large_scale Isolation and Purification of R_phycoerythrin from Red Alga Palmaria palmata Using the Expanded Bed Adsorption Method被引量:2
《Acta Botanica Sinica》2002年第5期541-546,共6页王广策 孙海宝 范晓 曾呈奎 
王宽诚基金资助项目 (2 0 0 0~ 2 0 0 1);中国科学院海洋研究所创新项目。~~
R-phycoerythrin, a light-harvesting protein in some marine algae, and can be widely used in medicine, was isolated and purified from a red alga, Palmaria palmata (Lannaeus) Kuntze, using the streamline column (expande...
关键词:Palmaria palmata ( Lannaeus) Kuntze R-PHYCOERYTHRIN streamline column ion-exchange chromatography 
Synthesis and energy transfer of model conjugate of phycobilisome rods
《Chinese Science Bulletin》1999年第2期134-139,共6页Jiquan Zhao Jingquan Zhao Lijin Jiang Jingmin Zhang Fuli Zhao Xiguang Zheng Hezhou Wang 
The conjugate of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) was synthesized through a heterobifunctional coupling reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. The molar ratio of R-PE to C-PC was determ...
关键词:R-PHYCOERYTHRIN C-PHYCOCYANIN rod MODEL of PHYCOBILISOMES energy transfer time-resolved fluorescence spectra 
Isolation, properties and spatial site analysis of γ subunits of B-phycoerythrin and R-phycoerythrin被引量:3
《Science China(Life Sciences)》1998年第1期9-17,共9页王广策 周百成 曾呈奎 
Polysiphonia urceolata R phycoerythrin and Porphyridium cruentum B phycoerythrin were degraded with proteinaseK, and then the nearly native γ subunits were isolated from the reaction mixture. The process of degradati...
关键词:B PHYCOERYTHRIN R PHYCOERYTHRIN γ SUBUNIT SPECTROSCOPIC properties. 
Synthesis and characterization of model conjugate of phycobilisomes
《Chinese Science Bulletin》1997年第17期1440-1444,共5页Zhao, JQ Zhao, JQ Yang, ZX Zhang, JP Jiang, LJ 
PHYCOBILISOMES are light-harvesting protein-pigment complexes located on the cytoplasmic
关键词:MODEL of PHYCOBILISOMES R-PHYCOERYTHRIN C-PHYCOCYANIN ALLOPHYCOCYANIN energy transfer. 
Combination of phase information from isomorphous crystal of R-phycoerythrin from Polysiphonia Urceolata
《Progress in Natural Science:Materials International》1996年第4期62-68,共7页WAN Zhuli CHANGWenrui JIANGTao SONG Haiwei ZHANG Jiping WANG Shuguang LIANG Dongcai 
supported by the Chinese Academy of Sciences;the National Natural Science Foundation of China.
On the basis of two isoraorphous heavy atom derivatives of R-phycoerythrin(R-PE),both new derivatives of R-PE have also been obtained m 0.05mol/L sodium phosphate buffer and(NH4)2SO4 system containing k2PtCl4(PTC4)and...
关键词:R-PHYCOERYTHRIN heavy atom derivatives phase information. 
Langmuir-Blodgett films of phycobiliproteins (II) --Photoelectrochemical property of R-phycoerythrin
《Science China Chemistry》1996年第4期386-397,共12页何靳安 蒋丽金 毕只初 江龙 
Project supported by the National Natural Science Foundation of China
Through an electrochemical cell deposited with R-phycoerythrin (R-PE) monolayer on SnO2 optically transparent electrode (SnO2 OTE), charge transport phenomenon and the photoelectrochemical behavior of R-PE have been i...
关键词:R-PHYCOERYTHRIN LB films PHOTOELECTRIC effect ELECTRON transfer. 
Langmuir-Blodgett films of phycobiliproteins (I)——Monomolecular film and conformational studies of R-phycoerythrin被引量:1
《Science China Chemistry》1996年第3期327-336,共10页何靳安 蒋丽金 江龙 毕只初 李津如 
Project supported by the National Natural Science Foundation of China
The surface pressure-area (π-A) isotherm of R-phycoerythrin (R-PE) at the air-water interface has been measured. The results indicate that R-PE can form the monomolecular film. Moreover, the molecule-occupied area ex...
关键词:R-PHYCOERYTHRIN MONOLAYER ELLIPSOMETRY LB technique TEM CONFORMATION of protein. 
SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS
《Chinese Journal of Oceanology and Limnology》1996年第1期36-40,共5页高洪峰 纪明侯 曹文达 
R-phycoerythrin from Porphyra haitanensis exists in two aggregaion states with different molecularweights. A more highly aggregated form, RPE I,Was chromatographed on Bio-Rex 70 column with ureasolution (pH 3.0) as ...
关键词:PORPHYRA haitanensis R-phycoerythnn SUBUNIT chromophote 
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