Langmuir-Blodgett films of phycobiliproteins (II) --Photoelectrochemical property of R-phycoerythrin  

作　　者：何靳安 蒋丽金 毕只初 江龙 

机构地区：[1]Institute of Photographic Chemistry, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Science China Chemistry》1996年第4期386-397,共12页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China

摘　　要：Through an electrochemical cell deposited with R-phycoerythrin (R-PE) monolayer on SnO2 optically transparent electrode (SnO2 OTE), charge transport phenomenon and the photoelectrochemical behavior of R-PE have been investigated. The experimental results indicate that the cell is able to generate photocurrent; moreover, the signal increases apparently in the presence of electron donor or acceptor in the electrolyte solution, showing that the photocurrent of R-PE would originate from its charge transfer. Further comparative test showed that the photocurrent came from the photo-induced charge separation property of the chromorphores attached covalently to the apoprotein of R-PE. The photocurrent spectrum of R-PE LB films verified the above viewpoint, from which the mechanism of photo-induced charge transfer of R-PE is suggested. The quantum yield for photoelectric conversion of R-PE LB films was measured to be φ520nm=3.4% and the photovoltage approached 400 mV. Moreover, the protein is stable for a long irradiation time. In addition, the effects of pH value of the electrolyte solution, the concentration of electron donor, the number of layers of R-PE LB films and the irradiation time on the photocurrent are studied.Through an electrochemical cell deposited with R-phycoerythrin (R-PE) monolayer on SnO2 optically transparent electrode (SnO2 OTE), charge transport phenomenon and the photoelectrochemical behavior of R-PE have been investigated. The experimental results indicate that the cell is able to generate photocurrent; moreover, the signal increases apparently in the presence of electron donor or acceptor in the electrolyte solution, showing that the photocurrent of R-PE would originate from its charge transfer. Further comparative test showed that the photocurrent came from the photo-induced charge separation property of the chromorphores attached covalently to the apoprotein of R-PE. The photocurrent spectrum of R-PE LB films verified the above viewpoint, from which the mechanism of photo-induced charge transfer of R-PE is suggested. The quantum yield for photoelectric conversion of R-PE LB films was measured to be φ520nm=3.4% and the photovoltage approached 400 mV. Moreover, the protein is stable for a

关 键 词：R-PHYCOERYTHRIN LB films PHOTOELECTRIC effect ELECTRON transfer. 

分 类 号：TB43[一般工业技术]