Langmuir-Blodgett films of phycobiliproteins (I)——Monomolecular film and conformational studies of R-phycoerythrin  

作　　者：何靳安 蒋丽金 江龙 毕只初 李津如 

机构地区：[1]Institute of Photographic Chemistry, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Science China Chemistry》1996年第3期327-336,共10页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China

摘　　要：The surface pressure-area (π-A) isotherm of R-phycoerythrin (R-PE) at the air-water interface has been measured. The results indicate that R-PE can form the monomolecular film. Moreover, the molecule-occupied area extrapolating the linear part of the n-A isotherm is identical with that when an R-PE molecule is located at the interface with its disk plane parallel to the air-water interface. The transmission electron micrograph (TEM) and the measurement of the thickness of the protein monolayer by ellipsometry show that the orientation of R-PE disk plane on the substrate is parallel to the plane of substrate. Absorption and fluorescence spectra of R-PE LB multilayers were obtained through transferring R-PE monolayer at the air-water interface to the substrates at the proper surface pressure by Langmuir-Blodgett (LB) technique. These spectra of R-PE LB films do not show distinct differences from those in aqueous solution. Comparative studies of circular dichroism (CD) spectra of the protein between in aqueous solution and in LB film show that the changes of the secondary structure of R-PE take place, i.e. the β-sheet component of the protein increases in the LB films.The surface pressure-area (π-A) isotherm of R-phycoerythrin (R-PE) at the air-water interface has been measured. The results indicate that R-PE can form the monomolecular film. Moreover, the molecule-occupied area extrapolating the linear part of the n-A isotherm is identical with that when an R-PE molecule is located at the interface with its disk plane parallel to the air-water interface. The transmission electron micrograph (TEM) and the measurement of the thickness of the protein monolayer by ellipsometry show that the orientation of R-PE disk plane on the substrate is parallel to the plane of substrate. Absorption and fluorescence spectra of R-PE LB multilayers were obtained through transferring R-PE monolayer at the air-water interface to the substrates at the proper surface pressure by Langmuir-Blodgett (LB) technique. These spectra of R-PE LB films do not show distinct differences from those in aqueous solution. Comparative studies of circular dichroism (CD) spectra of the protein between in

关 键 词：R-PHYCOERYTHRIN MONOLAYER ELLIPSOMETRY LB technique TEM CONFORMATION of protein. 

分 类 号：TB43[一般工业技术]