Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface  

作　　者：Langchong He Guangde Yang Xindu Geng 

机构地区：[1]NW Univ Xian, Inst Modern Separat Sci, Xian 710069, Peoples R China [2]Xian Med Univ, Coll Pharm, Xian 710061, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第9期826-831,共6页

摘　　要：A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system wouldA new cell membrane preparation. silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the absorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbi red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would be expected to indicate the special interaction and enantioselectivity between drugs and membrane receptorsin vivo.

关 键 词：cell membrane preparation silica CCM ENZYMATIC activity AFFINITY chromatography enantiomers. 

分 类 号：R341[医药卫生—基础医学]