DNA-based immunization against hepatitis B surface antigen carrying preS epitopes  

作　　者：Jingyi Hui Guangdi Li Yuying Kong Yuan Wang 

机构地区：[1]Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第7期620-623,共4页

摘　　要：Three plasmid expression vectors containing modified hepatitis B surface antigen (HBsAg) carrying pres epitopes were constructed. Transient expression after in vitro transfection in COS-M6 cells showed that under the transcriptional control of the human cytomegalovirus (CMV) immediate early promoter, fusion genes expressed the modified HBV envelope proteins which were efficiently secreted into culture medium and presented HBsAg, preS1 and preS2 antigenicity. DNA-based immunization with these plasmids carrying pres sequences induced anti-HBs antibody in BALB/c mice. The titers of anti-HBs antibody were higher than those appeared in mice immunized with plasmid carrying S gene only. DNA injection with plasmids containing preS1 sequences elicited also high titers of anti-preS1 antibody. Moreover, the antipreS1 antibodies were found to appear earlier than anti-HBs antibodies.Three plasmid expression vectors containing modified hepatitis B surface antigen (HBsAg) carrying preS epitopes were constructed. Transient expression afterin vitro transfection in COS-M6 cells showed that under the transcriptional control of the human cytomegalovirus (CMV) immediate early promoter, fusion genes expressed the modified HBV envelope proteins which were efficiently secreted into culture medium and presented HBsAg, preS1 and preS2 antigenicity. DNA-based immunization with these plasmids carrying preS sequences induced anti-HBs antibody in BALB/c mice. The titers of anti-HBs antibody were higher than those appeared in mice immunized with plasmid carrying S gene only. DNA injection with plasmids containing preSl sequences elicited also high titers of anti-preS1 antibody. Moreover, the antipreSl antibodies were found to appear earlier than anti-HBs antibodies.

关 键 词：PRES EPITOPES hepatitis B surface antigen (HBsAg) DNA-based IMMUNIZATION antibody. 

分 类 号：R392[医药卫生—免疫学]