Agar coating in multicellular spheroids culture of rat hepatocytes  

作　　者：Shaolin Li Jilun Pan Tao Xu Yaoting Yu 

机构地区：[1]Nankai Univ, Inst Mol Biol, Bioact Mat Res Lab, Tianjin 300071, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第24期2246-2249,共4页

摘　　要：Aggregation of freshly isolated adult rat hepatocytes in vitro is important for the construction of artificial liver support system. In the experiment, agar has been used as an extracellular matrix substrate and results demonstrate that hepatocytes in serum-free cultureAggregation of freshly isolated adult rat hepatocytesin vitro is important for the construction of artificial liver support system. In the experiment, agar has been used as an extracellular matrix substrate and results demonstrate that hepatocytes in serum-free culture medium can effectively form floating spheroids in tissue culture dishes coated with agar, which maintain higher ability to produce urea and albumin than monolayer cultured cells.

关 键 词：AGAR HEPATOCYTE multicellular spheroids. 

分 类 号：Q813[生物学—生物工程]