激光共聚焦显微镜研究大白鼠肝癌FSK7902细胞系的实体瘤中角蛋白和嗜铬颗粒素A的关系  

RELATIONSHIP OF CYTOKERATIN AND CHROMOGRANIN A ON THE CANCER CELLS OF SOLID TUMOR OF THE RAT HEPATOMA CELL LINE FSK 7902 WITH LASER SCANNING CONFOCAL MICROSOPY

作　　者：王春梅[1,2,3] 黄晓峰[1,2,3] 戴晓汶[1,2,3] 赵一岭[1,2,3] 苏勤 

机构地区：[1]第四军医大学基础部 [2]第四军医大学唐都医院病理科 [3]解放军117医院病理科

出　　处：《中国组织化学与细胞化学杂志》1999年第2期52-56,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：应用免疫组织化学ＡＢＣ法和免疫荧光组织化学方法结合激光共聚焦显微镜观察了嗜铬颗粒素Ａ和角蛋白在大白鼠肝癌ＦＳＫ７９０２细胞系形成的实体瘤癌细胞中的表达，结果表明在ＦＳＫ７９０２实体瘤内，大多数癌细胞呈角蛋白和ＣｇＡ免疫反应阳性，反应产物位于核周胞质或核旁的某些区域。Using the immunohistochemical ABC method and laser scanning confocal microscopy, the expression of cytokeratin and chromogranin A in the cancer cells of solid tumor of the rat hepatoma cell line FSK 7902 was investigated. The results showed that the cytokeratin and chromogranin A immunoreactive substances were existed in the most of the tumor cells. The immunoreactive substances localized in the perinuclear cytoplasms or nuclear surroundiug areas of tumor cells.

关 键 词：角蛋白 嗜铬颗粒素 免疫组织化学 激光扫描共聚焦显微镜 大鼠 

分 类 号：R735.703.11[医药卫生—肿瘤] R735.703[医药卫生—临床医学]