丙肝病毒抗原在肝细胞癌组织中的定位——共聚焦激光扫描显微镜观察  

LOCALIZATION OF HEPATITIS C VIRUS ANTIGEN IN HEPATOCELLULAR CARINOMA OBSERVATION WITH CONFOCA LASER SCANNING MICROSCOPE

作　　者：张王海[1,2] 朱世能[1,2] 陆世伦[1,2] 黄娅林[1,2] 赵鹏[1,2] 

机构地区：[1]上海医科大学病理学教研室 [2]上海医科大学医学神经生物学国家重点实验室

出　　处：《中国组织化学与细胞化学杂志》1999年第2期97-101,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金

摘　　要：应用共聚焦激光扫描显微镜（ＣｏｎｆｏｃａｌＬａｓｅｒＳｃａｎｎｉｎｇＭｉｃｒｏｓｃｏｐｅ，ＣＬＳＭ）观察免疫荧光标记的丙型肝炎病毒核心抗原（ＨＣＶＣＰ－１０）在肝癌组织中的表达，与免疫组化普通光镜观察结果比较，显示ＨＣＶＣＰ－１０抗原在肝细胞癌和癌周肝组织的定位特点，介绍ＣＬＳＭ在病理学中的应用。研究结果表明：ＨＣＶＣＰ－１０抗原大部分存在于胞浆，少部分存在于胞核，ＨＣＶ的表达有核内过程；ＨＣＶＣＰ－１０抗原在癌周肝组织的表达明显强于癌组织；The expression of HCV antigen labelled immunofluorescently in hepatocellular carcinoma was observed with confocal laser scanning microscope (CLSM), and the results were compared with that observed under conventional microscope immunohistochemically to investigate the application of CLSM in the field of pathology. The results indicated that HCV antigen was located largely in the cytoplasm of hepatocytes and HCC cells but less postive signals were seen in nuclei. These findings suggest that there are nuclear events during HCV antigen expression, and CLSM is more helpful than conventional microscope in localizing antigens.

关 键 词：肝细胞癌 丙型肝炎病毒 共聚焦激光扫描显微镜 

分 类 号：R735.703.11[医药卫生—肿瘤]