人食管鳞状上皮癌糖复合物表达的研究  

EXPRESSION ON GLYCOCONJUGATES IN SQUAMUS CARCINOMAS OF HUMAN ESOPHAGUS

作　　者：吴晓华[1,2] 徐小虎[1,2] 沈忠英[1,2] 杨家齐[1,2] 

机构地区：[1]汕头大学医学院法医学研究所 [2]中山医科大学法医学系法医病理学教研室

出　　处：《中国组织化学与细胞化学杂志》1999年第2期102-107,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：凝集素可与其相对应的糖复合物特异性结合。在癌症形成过程中细胞表面经历着显著的变化，本文用十种生物素化凝集素即ＵＥＡ－Ｉ、ＲＣＡ－Ｉ、ＤＢＡ、ＰＳＡ、ＰＮＡ、ＢＳＬ、ＬＣＡ、ＷＧＡ、ＣｏｎＡ和ＳＢＡ作为探针，对正常食管上皮和食管鳞状上皮癌进行研究，以确定正常食管上皮和食管鳞状上皮癌中糖复合物的改变，结果发现，ＰＮＡ和ＰＳＡ在正常食管和食管鳞状上皮癌中均无反应，ＲＣＡ－Ｉ、ＤＢＡ、ＢＳＬ、ＬＣＡ、ＣｏｎＡ和ＳＢＡ受体在正常和癌组织中有着特征性变化，ＢＳＬ和ＤＢＡ在食管中无反应，ＬＣＡ、ＳＢＡ和ＲＣＡ－１正常食管和食管鳞状上皮癌中反应方式不同，ＣｏｎＡ和ＷＧＡ则在同一癌组织的不同部位都显示出不同的反应。尽管ＵＥＡ－Ｉ在正常食管和食管鳞状上皮癌均呈阳性反应，但似乎未表现出有意义的变化。上述结果提示食管癌的发生与含α－Ｄ－ＧａｌＮＡｃ（ＤＢＡ和ＳＢＡ）、β－Ｄ－Ｇａｌ／β－Ｎ－ａｃｅｔｙｌ－Ｄ－Ｇａｌａｃｔｏｓａｍｉｎｅ（ＲＣＡ－Ｉ）、α－Ｄ－Ｇｌｃ／α－Ｄ－Ｍａｎ（ＬＣＡ和ＣｏｎＡ）、［β－（１－４）－Ｄ－ＧｌｃＮＡｃ］２／ＮｅｕＮＡｃ（ＷＧＡ）和α－Ｄ－Ｇａｌ（ＢＳＬ）等的糖复合物的改变有较为密切的关系。Lectin can bind specifically to certain glycoconjugates. During the process of carcinogenesis, cell surface undergoes remarkable changes. We used 10 extensive biotinylated lectins as probes to evaluate differences between normal esophageal epithelium and esophageal carcinoma. The results showed that PNA and PSA had no reactivity both in normal esophagus and esophageal carcinomas. Significant changes were found in BSL, LCA, DBA, SBA, ConA and RCA I. BSL and DBA had no reactivity in carcinoma, LCA, SBA, and RCA I had different reactive styles between normal esophagus and esophageal carcinoma, ConA and WGA showed characteristic stain pattern even in the different areas of the same carcinoma. Although UEA I had positive reactivity both in normal esophagus and esophageal carcinoma, it appeared that they had no significant changes. These results indicate that the changes of glycoconjugates containing α D GalNAc(DBA and SBA), β D Gal/β N-acetyl D Galactosamine(RCA I), α D Glc/α D Man(LCA and ConA), [β (1 4) D GlcNAc]2/NeuNAc(WGA) and α D GalNAc(BSL) may involve in the development of esophageal carcinoma.

关 键 词：食管上皮 食管癌 糖复合物 凝集素 亲和组织化学 

分 类 号：R735.1[医药卫生—肿瘤]