EXPERIMENTAL STUDY OF INFLUENCE OF LIQUID NITROGEN PRESERVATION ON ANTIGENICITY OF HUMAN HOMOGRAFT AORTIC VALVE  

作　　者：Fan Zhaohui,Huang Qingheng,Zhao Jian, Geng Xigang,Liu Jianlin,Ren Huping Department of Cardiovascular Surgery, First Affiliated Hospital of Xi′an Medical University, Xi′an 710061 

出　　处：《Journal of Pharmaceutical Analysis》1999年第2期157-161,共5页药物分析学报（英文版）

摘　　要：To illustrate the effect of liquid nitrogen preservation on antigenicity of human homograft aortic valve (HAV), human aortic valve tissue were cocultured together with peripheral blood mononuclear cells (PBMCs). Following detections were done to show the difference of antigenicity of HAV indirectly at different period after being preserved in liquid nitrogen: ① 3H TdR incorporation (cpm) was observed after tissue cell cocultured and stimulation index (SI) was calculated;② Density of IL 2 in medium was measured by MTT means. The results indicated that antigenicity of fresh HAV was the strongest; with the prolong of being preserved, antigenicity decreased gradually. It decreased significantly within first 48 h preserved at 4℃, then decreased significantly after preserved in liquid nitrogen for 24 h. 2 weeks later, antigenicity decreased to the lowest level.To illustrate the effect of liquid nitrogen preservation on antigenicity of human homograft aortic valve (HAV), human aortic valve tissue were cocultured together with peripheral blood mononuclear cells (PBMCs). Following detections were done to show the difference of antigenicity of HAV indirectly at different period after being preserved in liquid nitrogen: ① 3H TdR incorporation (cpm) was observed after tissue cell cocultured and stimulation index (SI) was calculated;② Density of IL 2 in medium was measured by MTT means. The results indicated that antigenicity of fresh HAV was the strongest; with the prolong of being preserved, antigenicity decreased gradually. It decreased significantly within first 48 h preserved at 4℃, then decreased significantly after preserved in liquid nitrogen for 24 h. 2 weeks later, antigenicity decreased to the lowest level.

关 键 词：liquid nitrogen homograft aortic valve (HAV) ANTIGENICITY stimulation index(SI) 

分 类 号：R392.4[医药卫生—免疫学]