Bcl-6在K562细胞的表达及其在髓系分化诱导机制中的作用  

Bcl-6 Expression in K562 Cells and Its Role in Mechanism Underlying Induced Differentiation into Various Myelocytic lineages

作　　者：张永清[1] 黄高昇[2] 陈协群[1] 白庆咸[1] 梁蓉[1] 王哲[2] 

机构地区：[1]第四军医大学西京医院血液科,陕西西安710032 [2]第四军医大学西京医院病理科,陕西西安710032

出　　处：《中国实验血液学杂志》2009年第2期290-293,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号30171186

摘　　要：本研究探讨诱导K562细胞分别向髓系不同细胞系分化时bcl-6表达的变化,并分析其机制。用Western blot检测以十四烷酸佛波醇-13-乙酯(tetradecanoylphorbol13-acetate,TPA)、羟基脲(hydooxyurea,Hu)及六甲基双乙酰胺(hexamethylene bisacetamide,HMBA)为诱导剂分别诱导K562细胞向巨核细胞系、红细胞系及巨噬细胞系分化时bcl-6表达变化,用PCR、克隆、直接DNA测序方法分析bcl-6基因5′调控区序列变异情况。结果表明,BCL-6蛋白仅在TPA诱导K562细胞向巨核细胞系分化时表达上调,其5′调控区序列未发现有突变发生。结论:bcl-6可能是调控K562细胞向髓系不同细胞系分化的关键基因之一,在TPA诱导的K562细胞向巨核细胞分化过程中发挥重要作用,其表达上调不伴有调控区基因突变。This study was purposed to investigate the changes of bcl-6 expression in K562 cells and the mechanism inducing differentiation into different myelocyte lineages. Models of K562 cells inducing differentiation to lineages of megakaryocyte, erythrocyte and macrophagocyte were established with inducers TPA （tetradecanoylphorbol 13-acetate）, Hu （hydrozyurea） and HMBA （hexamethylene bisacetamide） respectively. Western blot assay was applied to detect the expression of bcl-6 in K562 cells before and after the induction. Meanwhile, PCR, cloning and direct DNA sequencing were used to identify mutations in the 5′regulatory region of bcl-6 in K562 cells before and after induction with TPA. The results indicated that up-regulation of bcl-6 expression was found only in K562 cells being induced differentiating into megakaryocyte-lineage, while mutation of 5′regulatory region of bcl-6 gene was not found. It is concluded that ex- pression of bcl-6 increases only when K562 cells differentiate into megakaryocyte lineage and bcl-6 expression may play an important role in K562 cells induced differentiation into megakaryocyte lineage. The up-regulation of bcl-6 expression may not be related with the mutation of 5′regulatory regions of the gene.

关 键 词：K562细胞 BCL-6基因 细胞分化 髓系细胞 TPA 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]