三色流式细胞术分析急性淋巴细胞白血病免疫分型  

Analysis of Immunophenotypes of Acute Lymphoblastic Leukemia by Three Color Flow Cytometry

作　　者：邱阳[1] 王刚[2] 刘越坚[1] 单路娟[1] 

机构地区：[1]大连医科大学附属第一医院中心实验室,辽宁大连116011 [2]辽宁出入境检验检疫局,辽宁大连116011

出　　处：《中国实验血液学杂志》2009年第2期442-444,共3页Journal of Experimental Hematology

摘　　要：本研究探讨急性淋巴细胞白血病(ALL)各亚型免疫分型的特点及其临床意义。利用CD45/SSC双参数散点图设门方法,对40例ALL进行三色流式细胞术免疫表型分析。结果显示:采用CD45/SSC双参数散点图设门法能清楚地区分骨髓或外周血中的淋巴细胞、单核细胞、粒细胞、有核红细胞和原始细胞群。40例ALL中B-ALL26例,T-ALL11例,HAL3例。26例B-ALL中CD19全部(100%)阳性表达,而11例T-ALL中CD7表达的阳性率最高(100%)。伴髓系抗原表达的ALL(My+ALL)比较常见,本实验中共有12例,约达到30.0%,经常累及B淋巴系统(占My+ALL的75.0%);在髓系抗原中以CD13表达阳性率最高;有3例患者为急性杂合性白血病(HAL),发病率为7.5%,且全部为髓系、B系共同表达,其中有2例患者CD34阳性表达,占66.67%。结论:在流式细胞仪上采用CD45/SSC双参数散点图设门方法进行三色流式细胞术分析,能排除正常细胞的干扰,使免疫分型的结果更为准确、可靠。This study was purposed to investigate the immunophenotype characteriscs and thier significance in subtypes of acute lymphoblastic leukemia （ALL）. The immunophenotypes in 40 cases of ALL were analyzed with three color flow cytometry using CD45/SSC two-parametric gating. The results showed that the three color flow cytometry assay using CD45/SSC two-parametric gating could accurately distinguish each other from lymphocytes, monocytes, granulo- cytes, erythroblasts and primitive cells in bone marrow and/or peripheral blood. Among 40 cases of ALL, B-ALL was 26 cases, T-ALL was 11 cases, HAL was 3 cases. All of the 26 cases of B-ALL expressed CD19 with positive rate of 100%, meanwhile 11 cases of T-ALL most highly expressed CD17 with positive rate of 100%. 12 cases of ALL with myeloid antigen expression （My-ALL） were involved in ALL, the incidence of these cases was almost 30% （12/40）. The CD13 was expressed most highly in myeloid antigens. All 3 cases of HAL coexpressed myeloid and B-lineage anti- gens, among them CD34 was expressed in 2 cases with positive rate of 66.67%. It is concluded that three color flow cytometry assay using CD45/SSC two-parametric gating can exclude the interference of normal cells, thereby the results are more reliable and more accurate.

关 键 词：急性淋巴细胞白细胞 免疫分型 流式细胞术 

分 类 号：R733.71[医药卫生—肿瘤]