重组葡激酶的基因克隆与序列测定  

Cloning and sequencing of recombinant staphylokinase

作　　者：李倩[1] 荫俊[1] 雷祚荣[1] 宋伟[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《生物技术通讯》1999年第3期180-182,共3页Letters in Biotechnology

摘　　要：从烧伤病房病人烧伤感染创面分离到 9株金黄色葡萄球菌 ,经纤溶活性测定 ,选纤溶活性最高的作为 PCR扩增模板。将扩增出的葡激酶基因片段插入 p UC1 8载体质粒 ,转化大肠杆菌 DH5 α,筛选出 PUC- SAK阳性克隆 ,经 DNA序列测定证实该基因片段为一种新的Nine Strains of Staphylococcus aureus were isolated from some patients who suffer from bacterial infection in a burn ward of a hospital. After detecting of fibrinolytic activity in all of the strains, select the strain with strong fibrinolytic activity as a template of PCR amplification. PCR products insert in cloning vector pUC18 and transformed into E.coli DH5α. Screen the positive cloning which contains the cDNA of staphylokinase, DNA sequencing demonstrated that it were novel.

关 键 词：重组葡激酶 基因克隆 序列测定 

分 类 号：Q785[生物学—分子生物学]